Sparing effect of procyanidins from Vitis vinifera on vitamin E: In vitro studies

The sparing/recycling effect of a highly purified, high molecular weight fraction of catechin oligomers (procyanidins) from Vitis vinifera seeds on alpha-tocopherol was studied in both homogeneous solution and in heterogeneous phase (phosphatidylcholine liposomes and red blood cells). By HPLC and electron spin resonance (ESR) spectroscopy we evidenced that tocopheroxyl radical, induced by reaction of alpha-tocopherol with the stable radical DPPH (2,2-diphenyl-1-picrylhydrazyl) is recycled by procyanidins. In addition procyanidins significantly and dose-dependently spare vitamin E from consumption (HPLC monitoring) during the autooxidation phase of the HO.-induced peroxidation of phosphatidylcholine, by 23% at the lowest concentration (0.5 mu M) and by 65.5 % at 3 mu M In this membrane model the combination of 0.5 mu M procyanidins and 2 mu M alpha-tocopherol results in a marked delay in the appearance of conjugated dienes in respect to the single antioxidants (synergistic interaction), while catechin showed to be active only at 5 mu M. In red blood cells oxidatively stressed by UVB exposure, procyanidins at 0.1-1.0 mu M prevent vitamin E loss, markedly decrease membrane lipid peroxidation, linearly related to the concentration of vitamin E in the membranes, and significantly delay the onset of hemolysis (catechin protects between 5 and 10 mu M).