Evaluation of mass transfer rate in aqueous two-phase systems: Effect of microchannel width for bovine serum albumin extraction

被引：25

作者：

Raji, Farshad ^{[1
]}

Rahbar-Kelishami, Ahmad ^{[1
]}

机构：

[1] Iran Univ Sci & Technol IUST, Fac Chem Petr & Gas Engn, Res Lab Adv Separat Proc, Tehran, Iran

来源：

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION | 2021年 / 163卷

关键词：

Aqueous two-phase systems; Biomolecules; Microfluidics; Bovine serum albumin (BSA); Microchannel width; Mass transfer; SEPARATION; PROTEINS; BEHAVIOR; FLOW;

D O I：

10.1016/j.cep.2021.108370

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

In this research, extraction of bovine serum albumin (BSA) with aqueous two-phase systems (ATPS) based on polyethylene glycol/sodium citrate using the microfluidic apparatus were investigated. The operational variables were studied using microchannel width (800-400 mu m), salt phase flow rate (1.6-4 mL/h), polyethylene glycolrich phase flow rate (0.8-2 mL/h), and pH (5-9) by response surface methodology (RSM). Double interfaces performed BSA transfer from the salt phase to the polyethylene glycol (PEG) phase. The effects of adding the interface on the mass transfer coefficient and the mass transfer rate were evaluated. Using the microfluidic system due to having two interfaces will increase the extraction rate compared to macro systems with a single interface. In values, salt phase pH of 7, PEG phase flow rate of 2 mL/h, and salt phase flow rate of 4 mL/h were the optimum condition for BSA extraction rate (70.9%.) in this study.

引用

页数：8

共 44 条

[1] Development of a microfluidic-chip system based on parallel flow for intensified Gd(III) extraction from nitrate media using cationic extractant [J].