Biosensor based on electrospun blended chitosan-poly (vinyl alcohol) nanofibrous enzymatically sensitized membranes for pirimiphosmethyl detection in olive oil

被引：46

作者：

El-Moghazy, A. Y. ^{[1
]}

Soliman, E. A. ^{[1
]}

Ibrahim, H. Z. ^{[2
]}

Marty, J. -L. ^{[3
]}

Istamboulie, G. ^{[3
]}

Noguer, T. ^{[3
]}

机构：

[1] City Sci Res & Technol Applicat SRTA City, Polymer Mat Res Dept, Adv Technol & New Mat Res Inst, Alexandria 21934, Egypt

[2] Univ Alexandria, Dept Environm Studies, Inst Grad Studies & Res, Alexandria 832, Egypt

[3] Univ Perpignan, BAE, Via Domitia,52 Ave Paul Alduy, F-66860 Perpignan, France

来源：

TALANTA | 2016年 / 155卷

关键词：

Biosensor; Nanofibers; Electrospinning; Genetically-Modified Acetylcholinesterase; Organophosphorus Pesticides; Olive Oil; ACETYLCHOLINESTERASE; ORGANOPHOSPHATE; IMMOBILIZATION; NANOCOMPOSITE; PESTICIDES;

D O I：

10.1016/j.talanta.2016.04.018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ultra-sensitive electrochemical biosensor was successfully developed for rapid detection of pirimiphos-methyl in olive oil, based of genetically-engineered acetylcholinesterase (AChE) immobilization into electrospun chitosan/poly (vinyl alcohol) blend nanofibers. Due to their unique properties such as spatial structure, high porosity, and large surface area, the use of nanofibers allowed improving the biosensor response by two folds. The developed biosensor showed a good performance for detecting pirimiphos-methyl, with a limit of detection of 0.2 nM, a concentration much lower than the maximum residue limit allowed set by international regulations (164 nM). The biosensor was used for the detection of pirimiphos-methyl in olive oil samples after a simple liquid-liquid extraction, and the recovery rates were close to 100%. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：258 / 264

页数：7

共 33 条

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