Effects of LPS and IL-6 on oxytocin receptor in non-pregnant and pregnant rat uterus

被引：42

作者：

Fang, X ^{[1
]}

Wong, S ^{[1
]}

Mitchell, BF ^{[1
]}

机构：

[1] Univ Alberta, Dept Obstet & Gynecol, Perinatal Res Ctr, Edmonton, AB T5H 3V9, Canada

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2000年 / 44卷 / 02期

关键词：

cytokines; estrogen; parturition; progesterone;

D O I：

10.1111/j.8755-8920.2000.440201.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

PROBLEM: Little is known regarding the regulation of the timing of parturition. Recent evidence suggests an interaction between the immune system and uterine contractility in late gestation. METHOD: Pregnant rats were treated with LPS in vivo in attempts to establish a model of premature parturition induced by the pro-inflammatory response. Uterine explants were incubated in vitro to determine the effects of IL-6 on uterine synthesis of oxytocin (OT) and its receptor (OTR). RESULTS: LPS injection was quite toxic to pregnant rats and gave extremely variable results. In animals that delivered, there was a marked increase in the uterine concentrations of OTR and OTR mRNA. There was no consistent effect regarding the timing of parturition. IL-6 caused a significant increase in the concentration of OTR mRNA in uterine explants from pregnant rats but not in tissues from non-pregnant animals. CONCLUSION: Rat uterine concentrations of OTR are regulated by IL-6. Pro-inflammatory cytokines may stimulate uterine contractility in late gestation rat uterine tissues through a mechanism involving stimulation of OTR.

引用

页码：65 / 72

页数：8

共 29 条

[1] NF-IL6 and NF-κB in cytokine gene regulation [J].

Akira, S ;

Kishimoto, T .

ADVANCES IN IMMUNOLOGY, VOL 65, 1997, 65 :1-46

[2] Interleukin-1 beta intra-amniotic infusion induces tumor necrosis Factor-alpha, prostaglandin production, and preterm contractions in pregnant rhesus monkeys [J].

Baggia, S ;

Gravett, MG ;

Witkin, SS ;

Haluska, GJ ;

Novy, MJ .

JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, 1996, 3 (03) :121-126

[3] TRANSFORMING GROWTH-FACTOR-BETA-2 PREVENTS PRETERM DELIVERY INDUCED BY INTERLEUKIN-1-ALPHA AND TUMOR-NECROSIS-FACTOR-ALPHA IN THE RABBIT [J].

BRY, K ;

HALLMAN, M .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1993, 168 (04) :1318-1322

[4] CACHECTIN TUMOR NECROSIS FACTOR-ALPHA FORMATION IN HUMAN DECIDUA - POTENTIAL ROLE OF CYTOKINES IN INFECTION-INDUCED PRETERM LABOR [J].

CASEY, ML ;

COX, SM ;

BEUTLER, B ;

MILEWICH, L ;

MACDONALD, PC .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (02) :430-436

[5] OXYTOCIN RECEPTOR SUBTYPES IN THE PREGNANT RAT MYOMETRIUM AND DECIDUA - PHARMACOLOGICAL DIFFERENTIATIONS [J].

CHAN, WY ;

CHEN, DL ;

MANNING, M .

ENDOCRINOLOGY, 1993, 132 (03) :1381-1386

[6] ESTROGEN STIMULATES OXYTOCIN GENE-EXPRESSION IN HUMAN CHORIO-DECIDUA [J].

CHIBBAR, R ;

WONG, S ;

MILLER, FD ;

MITCHELL, BF .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1995, 80 (02) :567-572

[7] AN EXAMINATION OF QUANTITATIVE RELATIONSHIP BETWEEN PROGESTERONE AND MAINTENANCE OF PREGNANCY [J].

CSAPO, AI ;

WIEST, WG .

ENDOCRINOLOGY, 1969, 85 (04) :735-&

[8] Involvement of NF-kappa B in the regulation of cyclooxygenase-2 protein expression in LPS-stimulated J774 macrophages [J].

DAcquisto, F ;

Iuvone, T ;

Rombola, L ;

Sautebin, L ;

DiRosa, M ;

Carnuccio, R .

FEBS LETTERS, 1997, 418 (1-2) :175-178

[9] DETECTION OF INTERLEUKIN-1, INTERLEUKIN-6, AND TUMOR-NECROSIS-FACTOR-ALPHA IN THE UTERUS DURING THE 2ND-HALF OF PREGNANCY IN THE MOUSE [J].

DE, M ;

SANFORD, TH ;

WOOD, GW .

ENDOCRINOLOGY, 1992, 131 (01) :14-20

[10] DECIDUAL CELL BIOSYNTHESIS OF INTERLEUKIN-6 - REGULATION BY INFLAMMATORY CYTOKINES [J].

DUDLEY, DJ ;

TRAUTMAN, MS ;

ARANEO, BA ;

EDWIN, SS ;

MITCHELL, MD .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1992, 74 (04) :884-889

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