Anti-obesity Action of Fermented Barley Extracts with Lactobacillus plantarum dy-1 and Associated MicroRNA Expression in High-fat Diet-induced Obese Rats

被引:11
作者
Zhang Jia Yan [1 ]
Xiao Xiang [1 ]
Dong Ying [1 ]
Zhou Xing Hua [1 ]
机构
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Fermented barley extract; Lactobacillus plantarum dy-1; Anti-obesity; Lipid metabolism; miRNAs; Microarray; LACTOBACILLUS-PLANTARUM DY-1; HEPATIC LIPID-ACCUMULATION; CITRIFOLIA L. JUICE; INSULIN-RESISTANCE; DOWN-REGULATION; BETA-GLUCAN; PROLIFERATION; LIVER; ACID; SUPPLEMENTATION;
D O I
10.3967/bes2019.095
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Objective To further explore associated effects of Lactobacillus plantarum dy-1 (LFBE) on obesity and lipid metabolism at the gene expression level, the expression of microRNAs (miRNAs) was investigated in the liver of high-fat diet (HFD) induced obese rats. Methods Three groups of animal models were established. Changes in miRNA expression in the liver of each group were analyzed by microarray and RT-qPCR, complemented by bioinformatics. Palmitate-induced hepatocellular carcinoma (HepG2) cells were used as a model to validate the test. Results LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride (TG) and total cholesterol (TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten miRNAs and downregulation of five miRNAs in the liver. Downregulation of miR-34a and miR-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes (KEGG) pathway analysis showed that possible target genes of the deregulated miRNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways. Conclusion These results demonstrate that LFBE might regulate the expression of miRNAs in order to inhibit obesity and fatty liver.
引用
收藏
页码:755 / +
页数:15
相关论文
共 33 条
  • [1] β-glucan from barley and its lipid-lowering capacity: a meta-analysis of randomized, controlled trials
    AbuMweis, S. S.
    Jew, S.
    Ames, N. P.
    [J]. EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2010, 64 (12) : 1472 - 1480
  • [2] Mechanism for HIF-1 activation by cholesterol under normoxia: A redox signaling pathway for liver damage
    Anavi, Sarit
    Hahn-Obercyger, Michal
    Madar, Zecharia
    Tirosh, Oren
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2014, 71 : 61 - 69
  • [3] How do microRNAs regulate gene expression?
    Cannell, Ian G.
    Kong, Yi Wen
    Bushell, Martin
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2008, 36 : 1224 - 1231
  • [4] Consumption of barley β-glucan ameliorates fatty liver and insulin resistance in mice fed a high-fat diet
    Choi, Joo Sun
    Kim, Hyunsook
    Jung, Myeong Ho
    Hong, Shinhee
    Song, Jihyun
    [J]. MOLECULAR NUTRITION & FOOD RESEARCH, 2010, 54 (07) : 1004 - 1013
  • [5] miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting
    Esau, C
    Davis, S
    Murray, SF
    Yu, XX
    Pandey, SK
    Pear, M
    Watts, L
    Booten, SL
    Graham, M
    McKay, R
    Subramaniam, A
    Propp, S
    Lollo, BA
    Freier, S
    Bennett, CF
    Bhanot, S
    Monia, BP
    [J]. CELL METABOLISM, 2006, 3 (02) : 87 - 98
  • [6] Differential Glucose-Regulation of MicroRNAs in Pancreatic Islets of Non-Obese Type 2 Diabetes Model Goto-Kakizaki Rat
    Esguerra, Jonathan Lou S.
    Bolmeson, Caroline
    Cilio, Corrado M.
    Eliasson, Lena
    [J]. PLOS ONE, 2011, 6 (04):
  • [7] Aberrantly elevated microRNA-34a in obesity attenuates hepatic responses to FGF19 by targeting a membrane coreceptor β-Klotho
    Fu, Ting
    Choi, Sung-E
    Kim, Dong-Hyun
    Seok, Sunmi
    Suino-Powell, Kelly M.
    Xu, H. Eric
    Kemper, Jongsook Kim
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (40) : 16137 - 16142
  • [8] MicroRNA-130b targets Fmr1 and regulates embryonic neural progenitor cell proliferation and differentiation
    Gong, Xi
    Zhang, Kunshan
    Wang, Yanlu
    Wang, Junbang
    Cui, Yaru
    Li, Siguang
    Luo, Yuping
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 439 (04) : 493 - 500
  • [9] Effect of miR-467b on atherosclerosis of rats
    Guan, Xiao-Mei
    Li, Yong-Xin
    Xin, Hai
    Li, Jun
    Zhao, Zong-Gang
    Wang, Yue-Wei
    Wang, Hao-Fu
    [J]. ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2016, 9 (03) : 293 - 296
  • [10] MicroRNA 421 suppresses DPC4/Smad4 in pancreatic cancer
    Hao, Jun
    Zhang, Shuyu
    Zhou, Yingqi
    Liu, Cong
    Hu, Xiangui
    Shao, Chenghao
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 406 (04) : 552 - 557