Effects of cigarette smoking and age on the maturation of human oocytes

We investigated whether cigarette smoking, measured by follicular fluid concentrations of cotinine (a major metabolite of nicotine), affects the maturity of oocytes from women undergoing in-vitro fertilization (IVF) and embryo transfer, In 233 women, follicular fluid samples were assessed for cotinine and their 2020 oocytes were assessed for maturity stage, Data on individual proportions of oocgtes which were mature (OM) and were fertilized (OF) mere analysed by regression in relation to age and follicular fluid cotinine, OF gave an independent assessment of oocyte maturity, Both age and follicular fluid cotinine entered the OM and OF regressions and mere significant, The age-adjusted regression coefficients for log cotinine were positive; greater cotinine concentrations usually accompanied greater OM and OF, The cotinine effect on OM was positive in younger women, but it became negative (decreased OM with increasing cotinine concentrations) in older women (greater than or equal to 40 Sears). We further found in older women an average reduction of similar to 50 % in the number of mature oocytes; this reduced number was lower than the number of embryos usually transferred, Smoking can reduce the number of mature oocytes even further, therefore risking a negative IVF-embryo transfer outcome, This may be the reason why the negative effects of smoking become clinically detectable in older women.