Splice Variants of Superoxide Dismutases in Rice and Their Expression Profiles under Abiotic Stresses

被引:9
作者
Saini, Ajay [1 ,2 ,3 ]
Rohila, Jai S. [4 ]
Govindan, Ganesan [1 ]
Li, Yong-Fang [1 ]
Sunkar, Ramanjulu [1 ]
机构
[1] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
[2] Bhabha Atom Res Ctr, Div Mol Biol, Mumbai 400085, Maharashtra, India
[3] Homi Bhabha Natl Inst, Mumbai 400094, Maharashtra, India
[4] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA
关键词
abiotic stress; alternative splicing; rice; splice variants; SODs; superoxide dismutases; OXIDATIVE STRESS; GENE-EXPRESSION; COPPER; PLANTS; ARABIDOPSIS; COMPLEXITY; LANDSCAPE; SEQUENCE; EXCESS; PCR;
D O I
10.3390/ijms22083997
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The superoxide dismutases (SODs) play vital roles in controlling cellular reactive oxygen species (ROS) that are generated both under optimal as well as stress conditions in plants. The rice genome harbors seven SOD genes (CSD1, CSD2, CSD3, CSD4, FSD1, FSD2, and MSD) that encode seven constitutive transcripts. Of these, five (CSD2, CSD3, CSD4, FSD1, and MSD) utilizes an alternative splicing (AS) strategy and generate seven additional splice variants (SVs) or mRNA variants, i.e., three for CSD3, and one each for CSD2, CSD4, FSD1, and MSD. The exon-intron organization of these SVs revealed variations in the number and length of exons and/or untranslated regions (UTRs). We determined the expression patterns of SVs along with their constitutive forms of SODs in rice seedlings exposed to salt, osmotic, cold, heavy metal (Cu+2) stresses, as well as copper-deprivation. The results revealed that all seven SVs were transcriptionally active in both roots and shoots. When compared to their corresponding constitutive transcripts, the profiles of five SVs were almost similar, while two specific SVs (CSD3-SV4 and MSD-SV2) differed significantly, and the differences were also apparent between shoots and roots suggesting that the specific SVs are likely to play important roles in a tissue-specific and stress-specific manner. Overall, the present study has provided a comprehensive analysis of the SVs of SODs and their responses to stress conditions in shoots and roots of rice seedlings.
引用
收藏
页数:19
相关论文
共 50 条
  • [1] Expression Profiles of Class A Rice Heat Shock Transcription Factor Genes Under Abiotic Stresses
    Liu, Ai-Ling
    Zou, Jie
    Zhang, Xian-Wen
    Zhou, Xiao-Yun
    Wang, Wen-Fang
    Xiong, Xing-Yao
    Chen, Li-Yun
    Chen, Xin-Bo
    JOURNAL OF PLANT BIOLOGY, 2010, 53 (02) : 142 - 149
  • [2] Genomic Survey of PEBP Gene Family in Rice: Identification, Phylogenetic Analysis, and Expression Profiles in Organs and under Abiotic Stresses
    Zhao, Chunyu
    Zhu, Mo
    Guo, Yanying
    Sun, Jian
    Ma, Wenhong
    Wang, Xiaoxue
    PLANTS-BASEL, 2022, 11 (12):
  • [3] Expression Profiles of Class A Rice Heat Shock Transcription Factor Genes Under Abiotic Stresses
    Ai-Ling Liu
    Jie Zou
    Xian-Wen Zhang
    Xiao-Yun Zhou
    Wen-Fang Wang
    Xing-Yao Xiong
    Li-Yun Chen
    Xin-Bo Chen
    Journal of Plant Biology, 2010, 53 : 142 - 149
  • [4] Insights into the Superoxide Dismutase Gene Family and Its Roles in Dendrobium catenatum under Abiotic Stresses
    Huang, Hui
    Wang, Hui
    Tong, Yan
    Wang, Yuhua
    PLANTS-BASEL, 2020, 9 (11): : 1 - 18
  • [5] EXPRESSION PROFILES OF STRESS RESPONSIVE GENES IN RICE (ORYZA SATIVA L.) UNDER ABIOTIC STRESSES
    Ranawake, Aloka Lanka
    Mori, Naoki
    Nakamura, Chiharu
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2012, 26 (02) : 2838 - 2843
  • [6] Expression profiles of stress responsive genes in rice (Oryza sativa L.) under abiotic stresses
    Nakamura, C. (nakamura@kobe-u.ac.jp), 1600, Diagnosis Press Limited. (26): : 2838 - 2843
  • [7] Abiotic Stresses Cause Differential Regulation of Alternative Splice Forms of GATA Transcription Factor in Rice
    Gupta, Priyanka
    Nutan, Kamlesh K.
    Singla-Pareek, Sneh L.
    Pareek, Ashwani
    FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [8] Ethylene Modulates Rice Root Plasticity under Abiotic Stresses
    Qin, Hua
    Xiao, Minggang
    Li, Yuxiang
    Huang, Rongfeng
    PLANTS-BASEL, 2024, 13 (03):
  • [9] Genome-Wide Identification and Expression Analysis of RCC1 Gene Family under Abiotic Stresses in Rice (Oryza sativa L.)
    Cen, Qiwen
    Kang, Lihua
    Zhou, Danni
    Zhang, Xian
    Tian, Quanxiang
    Zhang, Xiaoqin
    Mou, Wangshu
    Dang, Cong
    Fang, Yunxia
    Xue, Dawei
    AGRONOMY-BASEL, 2023, 13 (03):
  • [10] Characterization of B-box family genes and their expression profiles under abiotic stresses in the Melilotus albus
    Nian, Lili
    Zhang, Xiaoning
    Liu, Xingyu
    Li, Xiaodan
    Liu, Xuelu
    Yang, Yingbo
    Haider, Fasih Ullah
    Zhu, Xiaolin
    Ma, Biao
    Mao, Zixuan
    Xue, Zongyang
    FRONTIERS IN PLANT SCIENCE, 2022, 13