Phagocytic Activity of Macrophages against Liposomes with Conjugates of Oxidized Dextrans and Isonicotinic Acid Hydrazide during Modeling of Phagocytosis Disturbances In Vitro

被引：0

作者：

Arkhipov, S. A. ^{[1
]}

Shkurupy, V. A. ^{[1
]}

Troitsky, A. V. ^{[1
]}

Luzgina, N. G. ^{[1
]}

Ufimceva, E. G. ^{[1
]}

Zaikovskaja, M. V. ^{[1
]}

Iljine, D. A. ^{[1
]}

Akhramenko, E. S. ^{[1
]}

Gulyaeva, E. P. ^{[1
]}

Bistrova, T. N. ^{[1
]}

机构：

[1] Russian Acad Med Sci, Res Ctr Clin & Expt Med, Siberian Div, Novosibirsk, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2009年 / 148卷 / 04期

关键词：

oxidized dextrans; isonicotinic acid hydrazide; liposomes; phagocytosis; macrophages;

D O I：

10.1007/s10517-010-0795-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied phagocytic activity of macrophages against molecular-liposome hybrid compositions consisting of liposomes (diameter 200-450 nm) containing oxidized dextrans with a molecular weight of 35 or 60 kDa conjugated with the basic antituberculosis preparation isonicotinic acid hydrazide (dextrazides) during modeling of various disturbances of endocytosis function of phagocytic cells in vitro. Preincubation of macrophages with trypsin, colchicine, or sodium azide did not change the parameters of adhesion of molecular-liposome hybrid compositions to macrophages. It was found that preincubation of cells with colchicine or sodium azide reduced parameters of phagocytosis of the molecular-liposome hybrid compositions; this reduction did not depend on the molecular weight of dextrans entering the composition of the molecular-liposome hybrid compositions.

引用

页码：689 / 691

页数：3

共 7 条

[1] AKEI H, 2009, AM J PHYSIOL-LUNG C, V291, P572
[2] Akopyan V. P., 2005, Eksperimental'naya i Klinicheskaya Farmakologiya, V68, P36
[3] Human monocytes differentiate into macrophages under the influence of human KPB-M15 conditioned medium
Gersuk, G
Hiraoka, A
Marr, KA
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2005, 299 (1-2) : 99 - 106
[4] Goldberg E. D., 1992, METHODS TISSUE CULTU
[5] SHKURUPY VA, 2008, B EKSP BIOL MED, V145, P123
[6] SHKURUPY VA, 2000, Patent No. 2143900
[7] SHKURUPY VA, 2008, B EKSP BIOL MED, V145, P120

← 1 →