Magnetic carbon nanotube labelling for haematopoietic stem/progenitor cell tracking

被引:31
作者
Gul, Hilal [2 ,3 ]
Lu, Weibing [2 ]
Xu, Peng [4 ]
Xing, James [2 ,5 ]
Chen, Jie [1 ,3 ,4 ]
机构
[1] Univ Alberta, Dept Biomed Engn, ECERF W6 019, Edmonton, AB T6G 2V4, Canada
[2] IntelligentNano Inc, Edmonton, AB T6G 2M9, Canada
[3] Univ Alberta, Dept Elect & Comp Engn, Edmonton, AB T6G 2V4, Canada
[4] Natl Inst Nanotechnol, Edmonton, AB T6G 2M9, Canada
[5] Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada
基金
加拿大健康研究院;
关键词
IN-VIVO TRACKING; UMBILICAL-CORD BLOOD; PERIPHERAL-BLOOD; STEM-CELLS; DELIVERY; FUNCTIONALIZATION; DNA; BIOCOMPATIBILITY; EXPRESSION; MECHANISM;
D O I
10.1088/0957-4484/21/15/155101
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Haematopoietic stem and progenitor cell (HSPC) research has significantly contributed to the understanding and harnessing of haematopoiesis for regenerative medicine. However, the methodology for real-time tracking HSPC in vivo is still lacking, which seriously restricts the progress of research. Recently, magnetic carbon nanotubes (mCNT) have generated great excitement because they have been successfully used as vehicles to deliver a lot of biomolecules into various cells. There is, however, no report about mCNT being used for tracking HSPC. In this paper, we investigated the uptake efficiency of fluorescein-isothiocyanate-labelled mCNT (FITC-mCNT) into HSPC and their effect on the cytotoxicity and differentiation of HSPC. We found that cellular uptake of FITC-mCNT was concentration-and time-dependent. The uptake of FITC-mCNT into HSPC reached up to 100% with the highest mean fluorescence (MF). More importantly, efficient FITC-mCNT uptake has no adverse effect on the cell viability, cytotoxicity and differentiation of HSPC as confirmed by colony-forming unit assay (CFU). In conclusion, the results reported here suggest the further tailoring of mCNT for their use in HSPC labelling/tracking in vivo or gene delivery into HSPC.
引用
收藏
页数:9
相关论文
共 38 条
[11]   Hematopoietic stem cells do not engraft with absolute efficiencies [J].
Camargo, FD ;
Chambers, SM ;
Drew, E ;
McNagny, KM ;
Goodell, MA .
BLOOD, 2006, 107 (02) :501-507
[12]   Amphiphilic helical peptide enhances the uptake of single-walled carbon nanotubes by living cells [J].
Chin, Shook-Fong ;
Baughman, Ray H. ;
Dalton, Alan B. ;
Dieckmann, Gregg R. ;
Draper, Rockford K. ;
Mikoryak, Carole ;
Musselman, Inga H. ;
Poenitzsch, Vasiliki Z. ;
Xie, Hui ;
Pantano, Paul .
EXPERIMENTAL BIOLOGY AND MEDICINE, 2007, 232 (09) :1236-1244
[13]   Medical progress: Hematopoietic stem-cell transplantation [J].
Copelan, EA .
NEW ENGLAND JOURNAL OF MEDICINE, 2006, 354 (17) :1813-1826
[14]   Hematopoietic progenitor cells from umbilical cord blood and from peripheral blood for subsequent in vivo tracking in a xenotransplant mouse model XXX [J].
Daldrup-Link, HE ;
Rudelius, M ;
Oostendorp, RAJ ;
Jacobs, VR ;
Simon, GH ;
Gooding, C ;
Rummeny, EJ .
ACADEMIC RADIOLOGY, 2005, 12 (04) :502-510
[15]   Protein electrochemistry at carbon nanotube electrodes [J].
Davis, Jason J. ;
Coles, Richard J. ;
Hill, H.Allen O. .
1997, Elsevier Science S.A., Lausanne, Switzerland (440) :1-2
[16]   Valproic Acid Increases CXCR4 Expression in Hematopoietic Stem/Progenitor Cells by Chromatin Remodeling [J].
Gul, Hilal ;
Marquez-Curtis, Leah A. ;
Jahroudi, Nadia ;
Lo, Jennifer ;
Turner, A. Robert ;
Janowska-Wieczorek, Anna .
STEM CELLS AND DEVELOPMENT, 2009, 18 (06) :831-838
[17]  
Hirsch A, 2002, ANGEW CHEM INT EDIT, V41, P1853, DOI 10.1002/1521-3773(20020603)41:11<1853::AID-ANIE1853>3.0.CO
[18]  
2-N
[19]   Nanotube molecular transporters: Internalization of carbon nanotube-protein conjugates into mammalian cells [J].
Kam, NWS ;
Jessop, TC ;
Wender, PA ;
Dai, HJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (22) :6850-6851
[20]   Carbon nanotubes as intracellular transporters for proteins and DNA: An investigation of the uptake mechanism and pathway [J].
Kam, NWS ;
Liu, ZA ;
Dai, HJ .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (04) :577-581