Magnetic carbon nanotube labelling for haematopoietic stem/progenitor cell tracking

被引：31
作者：
Gul, Hilal [2 ,3 ]
Lu, Weibing [2 ]
Xu, Peng [4 ]
Xing, James [2 ,5 ]
Chen, Jie [1 ,3 ,4 ]
机构：
[1] Univ Alberta, Dept Biomed Engn, ECERF W6 019, Edmonton, AB T6G 2V4, Canada
[2] IntelligentNano Inc, Edmonton, AB T6G 2M9, Canada
[3] Univ Alberta, Dept Elect & Comp Engn, Edmonton, AB T6G 2V4, Canada
[4] Natl Inst Nanotechnol, Edmonton, AB T6G 2M9, Canada
[5] Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada
来源：
NANOTECHNOLOGY | 2010年 / 21卷 / 15期
基金：
加拿大健康研究院;
关键词：
IN-VIVO TRACKING; UMBILICAL-CORD BLOOD; PERIPHERAL-BLOOD; STEM-CELLS; DELIVERY; FUNCTIONALIZATION; DNA; BIOCOMPATIBILITY; EXPRESSION; MECHANISM;
D O I：
10.1088/0957-4484/21/15/155101
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Haematopoietic stem and progenitor cell (HSPC) research has significantly contributed to the understanding and harnessing of haematopoiesis for regenerative medicine. However, the methodology for real-time tracking HSPC in vivo is still lacking, which seriously restricts the progress of research. Recently, magnetic carbon nanotubes (mCNT) have generated great excitement because they have been successfully used as vehicles to deliver a lot of biomolecules into various cells. There is, however, no report about mCNT being used for tracking HSPC. In this paper, we investigated the uptake efficiency of fluorescein-isothiocyanate-labelled mCNT (FITC-mCNT) into HSPC and their effect on the cytotoxicity and differentiation of HSPC. We found that cellular uptake of FITC-mCNT was concentration-and time-dependent. The uptake of FITC-mCNT into HSPC reached up to 100% with the highest mean fluorescence (MF). More importantly, efficient FITC-mCNT uptake has no adverse effect on the cell viability, cytotoxicity and differentiation of HSPC as confirmed by colony-forming unit assay (CFU). In conclusion, the results reported here suggest the further tailoring of mCNT for their use in HSPC labelling/tracking in vivo or gene delivery into HSPC.
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页数：9
相关论文
共 38 条
[1]   Covalently bonded adducts of deoxyribonucleic acid (DNA) oligonucleotides with single-wall carbon nanotubes: Synthesis and hybridization [J].
Baker, SE ;
Cai, W ;
Lasseter, TL ;
Weidkamp, KP ;
Hamers, RJ .
NANO LETTERS, 2002, 2 (12) :1413-1417
[2]   Chemically functionalized carbon nanotubes [J].
Balasubramanian, K ;
Burghard, M .
SMALL, 2005, 1 (02) :180-192
[3]   Hematopoietic stem cell gene therapy: selecting only the best [J].
Bank, A .
JOURNAL OF CLINICAL INVESTIGATION, 2003, 112 (10) :1478-1480
[4]   Applications of Carbon Nanotubes in Biotechnology and Biomedicine [J].
Bekyarova, Elena ;
Ni, Yingchun ;
Malarkey, Erik B. ;
Montana, Vedrana ;
McWilliams, Jared L. ;
Haddon, Robert C. ;
Parpura, Vladimir .
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2005, 1 (01) :3-17
[5]   Enzyme-coated carbon nanotubes as single-molecule biosensors [J].
Besteman, K ;
Lee, JO ;
Wiertz, FGM ;
Heering, HA ;
Dekker, C .
NANO LETTERS, 2003, 3 (06) :727-730
[6]   Biomedical applications of functionalised carbon nanotubes [J].
Bianco, A ;
Kostarelos, K ;
Partidos, CD ;
Prato, M .
CHEMICAL COMMUNICATIONS, 2005, (05) :571-577
[7]   From molecular biology to nanotechnology and nanomedicine [J].
Bogunia-Kubik, K ;
Sugisaka, M .
BIOSYSTEMS, 2002, 65 (2-3) :123-138
[8]   Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].
Bulte, JWM ;
Douglas, T ;
Witwer, B ;
Zhang, SC ;
Strable, E ;
Lewis, BK ;
Zywicke, H ;
Miller, B ;
van Gelderen, P ;
Moskowitz, BM ;
Duncan, ID ;
Frank, JA .
NATURE BIOTECHNOLOGY, 2001, 19 (12) :1141-1147
[9]   Iron oxide MR contrast agents for molecular and cellular imaging [J].
Bulte, JWM ;
Kraitchman, DL .
NMR IN BIOMEDICINE, 2004, 17 (07) :484-499
[10]   Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing [J].
Cai, D ;
Mataraza, JM ;
Qin, ZH ;
Huang, ZP ;
Huang, JY ;
Chiles, TC ;
Carnahan, D ;
Kempa, K ;
Ren, ZF .
NATURE METHODS, 2005, 2 (06) :449-454
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