A pH sensitive fluorescent cyanine dye for biological applications

被引：100
作者：
Briggs, MS [1 ]
Burns, DD [1 ]
Cooper, ME [1 ]
Gregory, SJ [1 ]
机构：
[1] Amersham Pharmacia Biotech, Drug Discovery, Forest Farm, Cardiff CF14 7YT, S Glam, Wales
来源：
CHEMICAL COMMUNICATIONS | 2000年 / 23期
关键词：
D O I：
10.1039/b007108k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A pentamethine cyanine dye has been synthesised that is fluorescent when protonated, becoming non-fluorescent upon proton abstraction. The probe has a pK(a) of 7.5, with observable changes in the fluorescent emission properties across a pH range of 6.0-8.0, therefore providing a useful probe for studying pH in biological media.
引用
收藏
页码：2323 / 2324
页数：2
相关论文
共 20 条
  • [1] ADIE E, 2000, SOC BIOM SCREEN 6 AN
  • [2] Aslam M., 1998, BIOCONJUGATION PROTE
  • [3] Color and constitution I Halochromism of anhydronium bases related to the cyanine dyes
    Brooker, LGS
    Sprague, RH
    Smyth, CP
    Lewis, GL
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1940, 62 : 1116 - 1125
  • [4] COOPER ME, 1999, FLUORESCENCE MICROSC, V3, P37
  • [5] Fabbrizzi L, 1999, EUR J INORG CHEM, P35
  • [6] García-Ochoa I, 1999, CHEM-EUR J, V5, P897, DOI 10.1002/(SICI)1521-3765(19990301)5:3<897::AID-CHEM897>3.0.CO
  • [7] 2-H
  • [8] GOOD V, 1956, PH MEASUREMENTS THEI
  • [9] Haugland RP., 1999, HDB FLUORESCENT PROB
  • [10] Controllable pH modulation of lanthanide luminescence by intramolecular switching of the hydration state
    Lowe, MP
    Parker, D
    [J]. CHEMICAL COMMUNICATIONS, 2000, (08) : 707 - 708
12