Transforming growth factor-β inhibits nephronectin-induced osteoblast differentiation

被引：21
作者：
Fang, Ling [1 ,2 ,4 ]
Kahai, Shireen [1 ,4 ]
Yang, Weining [3 ]
He, Chengyan [2 ]
Seth, Arun [1 ,4 ]
Peng, Chun [3 ]
Yang, Burton B. [1 ,4 ]
机构：
[1] Sunnybrook Hlth Sci Ctr, Sunnybrook Res Inst, Toronto, ON M4N 3M5, Canada
[2] Jilin Univ, China Japan Union Hosp, Jilin, Peoples R China
[3] York Univ, Dept Biol, N York, ON M3J 1P3, Canada
[4] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M5S 1A1, Canada
来源：
FEBS LETTERS | 2010年 / 584卷 / 13期
关键词：
Matrix; Nephronectin; TGF-beta; Gene expression; TGF-BETA; EXPRESSION; INTEGRIN; ADHESION; KIDNEY; REGION; GENE; POEM;
D O I：
10.1016/j.febslet.2010.04.074
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We found that the effect of TGF-beta on Npnt expression is associated with a change in cell morphology in a dose-dependent manner. Npnt-induced osteoblast differentiation was also inhibited by TGF-beta, which changed cell morphology from cuboidal to fibroblastic, an indication that osteoblast differentiation was disrupted. Furthermore, TGF-beta inhibited differentiation of osteoblasts transfected with various truncated Npnt constructs, suggesting that TGF-beta can exert a down-stream effect on Npnt function. Our results suggest that TGF-beta can inhibit osteoblast differentiation through various mechanisms. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：2877 / 2882
页数：6
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