Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion

被引:11
|
作者
Lai, Mong Sing
Seki, Masayuki
Ui, Ayako
Enomoto, Takemi
机构
[1] Tohoku Univ, Mol Cell Biol Lab, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan
[2] Tohoku Univ, 21st Century COE Program, Comprehens Res & Educ Ctr Planning Drug Evaluat &, Aoba Ku, Sendai, Miyagi 9808578, Japan
关键词
DNA replication; sister chromatid cohesion; Sgs1; Rmi1; Top3;
D O I
10.1038/sj.embor.7401000
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Saccharomyces cerevisiae RecQ-mediated genome instability (Rmi1) protein was recently identified as the third member of the slow growth suppressor 1-DNA topoisomerase III (Sgs1-Top3) complex, which is required for maintaining genomic stability. Here, we show that cells lacking RMI1 have a mitotic delay, which is partly dependent on the spindle checkpoint, and are sensitive to the microtubule depolymerizing agent benomyl. We show that rmi1 and top3 single mutants are defective in sister chromatid cohesion, and that deletion of SGS1 suppresses benomyl sensitivity and the cohesion defect in these mutant cells. Loss of RAD51 also suppresses the cohesion defect of rmi1 mutant cells. These results indicate the existence of a new pathway involving Rad51 and Sgs1-Top3-Rmi1, which leads to the establishment of sister chromatid cohesion.
引用
收藏
页码:685 / 690
页数:6
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