Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging

被引：17

作者：

Arai, Yoshiyuki ^{[1
,2
]}

Takauchi, Hiroki ^{[2
]}

Ogami, Yuhei ^{[2
]}

Fujiwara, Satsuki ^{[1
]}

Nakano, Masahiro ^{[1
,2
]}

Matsuda, Tomoki ^{[1
,2
]}

Nagai, Takeharu ^{[1
,2
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, 8-1 Mihogaoka, Ibaraki, Osaka 5670047, Japan

[2] Osaka Univ, Dept Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

来源：

ACS CHEMICAL BIOLOGY | 2018年 / 13卷 / 08期

关键词：

DIFFRACTION RESOLUTION LIMIT; MICROSCOPY; BREAKING; BARRIER; STORM;

D O I：

10.1021/acschembio.8b00200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.

引用

页码：1938 / 1943

页数：6

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