Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering

被引：95
作者：
Ahmadi, Parisa [1 ]
Nazeri, Niloofar [1 ]
Derakhshan, Mohammad Ali [2 ]
Ghanbari, Hossein [1 ,3 ]
机构：
[1] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Med Nanotechnol, Regenerat Nanomed Res Grp, Tehran, Iran
[2] Shiraz Univ Med Sci, Sch Adv Med Sci & Technol, Dept Med Nanotechnol, Shiraz, Iran
[3] Univ Tehran Med Sci, Tehran Heart Ctr, Res Ctr Adv Technol Cardiovasc Med, Tehran, Iran
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2021年 / 180卷
关键词：
Polyurethane; Chitosan; Carbon nanotube; Nanofibrous scaffold; Cardiac tissue engineering; CARBON NANOTUBES; CELL-PROLIFERATION; COMPOSITE FIBERS; HYBRID SCAFFOLDS; CHITOSAN; POLYURETHANE; CARDIOMYOCYTES; POLYPYRROLE; FABRICATION; MEMBRANE;
D O I：
10.1016/j.ijbiomac.2021.03.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide. Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered. In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning. A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM). Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay. The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed. The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium. (c) 2021 Published by Elsevier B.V.
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页码：590 / 598
页数：9
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