cDNA Library for Mining Functional Genes in Sedum alfredii Hance Related to Cadmium Tolerance and Characterization of the Roles of a Novel SaCTP2 Gene in Enhancing Cadmium Hyperaccumulation

被引：32
|
作者：
Liu, Mingying [1 ,2 ,3 ]
He, Xuelian [1 ,2 ]
Feng, Tongyu [1 ,2 ]
Zhuo, Renying [1 ,2 ]
Qiu, Wenmin [1 ,2 ]
Han, Xiaojiao [1 ,2 ]
Qao, Guirong [1 ,2 ]
Zhang, Dayi [4 ]
机构：
[1] State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China
[2] Chinese Acad Forestry, Key Lab Tree Breeding Zhejiang Prov, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China
[3] Zhejiang Chinese Med Univ, Sch Basic Med Sci, Hangzhou 310053, Zhejiang, Peoples R China
[4] Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China
来源：
ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2019年 / 53卷 / 18期
基金：
中国国家自然科学基金;
关键词：
FOX HUNTING SYSTEM; REACTIVE OXYGEN; STRESS TOLERANCE; HEAVY-METALS; ANTIOXIDANT ENZYMES; PROTEIN-KINASE; ROOT-GROWTH; PLANTS; EXPRESSION; FAMILY;
D O I：
10.1021/acs.est.9b03237
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Heavy metal contamination presents serious threats to living organisms. Functional genes related to cadmium (Cd) hypertolerance or hyperaccumulation must be explored to enhance phytoremediation. Sedum alfredii Hance is a Zn/Cd cohyperaccumulator exhibiting abundant genes associated with Cd hypertolerance. Here, we developed a method for screening genes related to Cd tolerance by expressing a cDNA-library for S. alfredii Hance. Yeast functional complementation validated 42 of 48 full-length genes involved in Cd tolerance, and the majority of them were strongly induced in roots and exhibited diverse expression profiles across tissues. Coexpression network analysis suggested that 15 hub genes were connected with genes involved in metabolic processes, response to stimuli, and metal transporter and antioxidant activity. The functions of a novel SaCTP2 gene were validated by heterologous expression in Arabidopsis, responsible for retarding chlorophyll content decrease, maintaining membrane integrity, promoting reactive oxygen species (ROS) scavenger activities, and reducing ROS levels. Our findings suggest a highly complex network of genes related to Cd hypertolerance in S. alfredii Hance, accomplished via the antioxidant system, defense genes induction, and the calcium signaling pathway. The proposed cDNA-library method is an effective approach for mining candidate genes associated with Cd hypertolerance to develop genetically engineered plants for use in phytoremediation.
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收藏
页码：10926 / 10940
页数：15
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