Enzymatic reverse FIA method for total phenols determination in urine samples

被引：11

作者：

Grunhut, Marcos ^{[1
]}

Palomeque, Miriam E. ^{[1
]}

Lista, Adriana G. ^{[1
]}

Fernandez Band, Beatriz S. ^{[1
]}

机构：

[1] Univ Nacl Sur, FIA Lab, Dept Chem, RA-8000 Bahia Blanca, Buenos Aires, Argentina

来源：

TALANTA | 2007年 / 71卷 / 04期

关键词：

reverse FIA method; polyphenol oxidase; urine samples; BENZENE METABOLITES; RAPID-DETERMINATION; POLYPHENOL OXIDASE; CRUDE EXTRACT; CHROMATOGRAPHY; CHLOROPHENOLS; HYDROQUINONE; BIOSENSOR; ELECTRODE; WORKERS;

D O I：

10.1016/j.talanta.2006.07.032

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reverse flow injection spectrophotometric enzymatic method is proposed to quantify total phenols in urine samples. The polyphenol oxidase (PPO; EC 1.14.18.1) obtained as a crude extract from sweet potato root (Ipomoea batatas) was used as enzymatic catalyze. The detection limit, the sample throughput and relative standard deviation were 7.7 mg I-1 of total phenols, 49 h(-1) and 0.9%, respectively. The method was applied to real samples and a recovery study was carried out in order to its validation. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1520 / 1523

页数：4

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