Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer

被引：76

作者：

Yoza, B ^{[1
]}

Arakaki, A ^{[1
]}

Maruyama, K ^{[1
]}

Takeyama, H ^{[1
]}

Matsunaga, T ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2003年 / 95卷 / 01期

关键词：

bacterial magnetic particles; dendrimer; hyperbranched; polyamidoamine; DNA extraction;

D O I：

10.1263/jbb.95.21

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial and artificial magnetic particles were modified using a polyamidoamine (PAMAM) dendrimer and outer shell amines determined. Bacterial magnetic particles were the most consistently modified. Transmission electron microscopic (TEM) analysis showed that the artificial magnetic particles were structurally damaged by the modification process including sonication. Furthermore, laser particle analysis of the magnetite also revealed damage. Small quantities of dendrimer-modified bacterial magnetic particles were used to extract DNA from blood. The efficiency of DNA recovery was consistently about 30 ng of DNA using 2-10 mug of dendrimer-modifled bacterial magnetite. This technique was fully automated using newly developed liquid handling robots and bacterial magnetic particles.

引用

页码：21 / 26

页数：6

共 18 条

[11]

NAKAMURA C, 1995, J BIOL CHEM, V270, P28392

[12] A magnetosome-specific GTPase from the magnetic bacterium Magnetospirillum magneticum AMB-1 [J].

Okamura, Y ;

Takeyama, H ;

Matsunaga, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (51) :48183-48188

[13] AN INTRODUCTION TO BIOMIMETICS - A STRUCTURAL VIEWPOINT [J].

SARIKAYA, M .

MICROSCOPY RESEARCH AND TECHNIQUE, 1994, 27 (05) :360-375

[14]

Schüler D, 1998, J BACTERIOL, V180, P159

[15] Bacterial magnetosomes:: microbiology, biomineralization and biotechnological applications [J].

Schüler, D ;

Frankel, RB .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 52 (04) :464-473

[16] STARBURST DENDRIMERS - MOLECULAR-LEVEL CONTROL OF SIZE, SHAPE, SURFACE-CHEMISTRY, TOPOLOGY, AND FLEXIBILITY FROM ATOMS TO MACROSCOPIC MATTER [J].

TOMALIA, DA ;

NAYLOR, AM ;

GODDARD, WA .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1990, 29 (02) :138-175

[17] Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1 [J].

Yang, CD ;

Takeyama, H ;

Tanaka, T ;

Matsunaga, T .

ENZYME AND MICROBIAL TECHNOLOGY, 2001, 29 (01) :13-19

[18] DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound [J].

Yoza, B ;

Matsumoto, M ;

Matsunaga, T .

JOURNAL OF BIOTECHNOLOGY, 2002, 94 (03) :217-224

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