Ultrasensitive Detection of Prostate-Specific Antigen and Thrombin Based on Gold-Upconversion Nanoparticle Assembled Pyramids

被引：75

作者：

Hao, Tiantian ^{[1
,2
]}

Wu, Xiaoling ^{[1
,2
]}

Xu, Liguang ^{[1
,2
]}

Liu, Liqiang ^{[1
,2
]}

Ma, Wei ^{[1
,2
]}

Kuang, Hua ^{[1
,2
]}

Xu, Chuanlai ^{[1
,2
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi 214122, Jiangsu, Peoples R China

来源：

SMALL | 2017年 / 13卷 / 19期

基金：

中国国家自然科学基金;

关键词：

RAMAN-SPECTROSCOPY; METAL-IONS; AMPLIFICATION; BIOMARKERS; PROTEIN; NANOSTRUCTURES; IMMUNOSENSOR; MOLECULES; RESONANCE; CHIRALITY;

D O I：

10.1002/smll.201603944

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Self-assembled nanostructures have been used for the detection of numerous cancer biomarkers. In this study, a gold-upconversion-nanoparticle (Au-UCNP) pyramid based on aptamers is fabricated to simultaneously detect thrombin and prostate-specific antigen (PSA) using surface-enhanced Raman scattering (SERS) and fluorescence, respectively. The higher the concentration of thrombin, the lower the intensity of SERS. PSA connected with the PSA aptamer leads to an increase in fluorescence intensity. The limit of detection of thrombin and PSA reaches 57 x 10(-18) and 0.032 x 10(-18) m, respectively. In addition, the pyramid also exhibits great target specificity. The results of human serum target detection demonstrate that the Au-UCNP pyramid is an excellent choice for the quantitative determination of cancer biomarkers, and is feasible for the early diagnosis of cancer.

引用

页数：6

共 49 条

[1] A chiroptical molecular sensor for ferrocene [J].

Agnes, Marco ;

Nitti, Andrea ;

Griend, Douglas A. Vander ;

Dondi, Daniele ;

Merli, Daniele ;

Pasini, Dario .

CHEMICAL COMMUNICATIONS, 2016, 52 (77) :11492-11495

[2] Tunable Enhancement of Raman Scattering in Graphene-Nanoparticle Hybrids [J].

Balasubramanian, Kannan ;

Zuccaro, Laura ;

Kern, Klaus .

ADVANCED FUNCTIONAL MATERIALS, 2014, 24 (40) :6348-6358

[3] A Chiroptical Probe for Sensing Metal Ions in Water [J].

Caricato, Marco ;

Leza, Nerea Jordana ;

Roy, Kinkini ;

Dondi, Daniele ;

Gattuso, Giuseppe ;

Shimizu, Linda S. ;

Griend, Douglas A. Vander ;

Pasini, Dario .

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2013, 2013 (27) :6078-6083

[4] Structural DNA Nanotechnology for Intelligent Drug Delivery [J].

Chao, Jie ;

Liu, Huajie ;

Su, Shao ;

Wang, Lianhui ;

Huang, Wei ;

Fan, Chunhai .

SMALL, 2014, 10 (22) :4626-4635

[5] Upconversion Nanoparticles: Design, Nanochemistry, and Applications in Theranostics [J].

Chen, Guanying ;

Qju, Hailong ;

Prasad, Paras N. ;

Chen, Xiaoyuan .

CHEMICAL REVIEWS, 2014, 114 (10) :5161-5214

[6] Electrochemical Immunosensors for Detection of Cancer Protein Biomarkers [J].

Chikkaveeraiah, Bhaskara V. ;

Bhirde, Ashwinkumar A. ;

Morgan, Nicole Y. ;

Eden, Henry S. ;

Chen, Xiaoyuan .

ACS NANO, 2012, 6 (08) :6546-6561

[7]

de la Rica R, 2012, NAT NANOTECHNOL, V7, P821, DOI [10.1038/nnano.2012.186, 10.1038/NNANO.2012.186]

[8] Ultrasensitive Detection of Low-Abundance Protein Biomarkers by Mass Spectrometry Signal Amplification Assay [J].

Du, Ruijun ;

Zhu, Lina ;

Gan, Jinrui ;

Wang, Yuning ;

Qiao, Liang ;

Liu, Baohong .

ANALYTICAL CHEMISTRY, 2016, 88 (13) :6767-6772

[9]

Frampton G. M., 2013, SMALL, V31, P1023

[10] Optical Aptasensors for the Analysis of the Vascular Endothelial Growth Factor (VEGF) [J].

Freeman, Ronit ;

Girsh, Julia ;

Jou, Amily Fang-ju ;

Ho, Ja-an Annie ;

Hug, Thomas ;

Dernedde, Jens ;

Willner, Itamar .

ANALYTICAL CHEMISTRY, 2012, 84 (14) :6192-6198

← 1 2 3 4 5 →