A multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed fish products

被引：33

作者：

Catanese, Gaetano ^{[1
]}

Manchado, Manuel ^{[1
]}

Fernandez-Trujillo, Alejandra ^{[2
]}

Infante, Carlos ^{[1
]}

机构：

[1] IFAPA Ctr El Toruno, Lab Identificac Especies Pesqueras & Acuicolas, Cadiz 11500, Spain

[2] Univ Malaga, Fac Ciencias, Dept Microbiol, E-29071 Malaga, Spain

来源：

FOOD CHEMISTRY | 2010年 / 122卷 / 01期

关键词：

Scomber; Mackerels; Control region; Multiplex-PCR; Processed food; Authentication; RESTRICTION SITE ANALYSIS; DNA CONTROL REGION; SPECIES IDENTIFICATION; GROUPER EPINEPHELUS; GENETIC IDENTIFICATION; COMMERCIAL FISH; RFLP ANALYSIS; CANNED TUNA; POLYMORPHISM; BONITO;

D O I：

10.1016/j.foodchem.2010.02.036

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, we have developed a novel multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed food. The method consists of two novel Scomber japonicus- (104 bp) and Scomber australasicus-specific (143 bp) amplicons, respectively, corresponding to the mitochondrial control region. It also includes the previously described Scomber colias-specific product (159 bp) corresponding to the 5S ribosomal DNA, the Scomber scombrus-specific fragment (123 bp) from the mitochondrial NADH dehydrogenase subunit 5, and finally a positive amplification control corresponding to the small 12S rRNA subunit (188 bp). The system was assayed in fresh samples as well as in a total of 40 commercial samples including 28 different canned products and 12 unprocessed fresh fillets. A positive identification was observed in all cases according to their commercial labelling. Overall, this methodology reveals as a potential molecular tool for direct application in the authentication of Scomber mackerels in the seafood industry. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：319 / 326

页数：8

共 50 条

[41] Identification of hake species (Merluccius genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences [J].

Quinteiro, J ;

Vidal, R ;

Izquierdo, V ;

Sotelo, CG ;

Chapela, MJ ;

Pérez-Martín, RI ;

Rehbein, H ;

Hold, GL ;

Russell, VJ ;

Pryde, SE ;

Rosa, P ;

Santos, AT ;

Rey-Méndez, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (11) :5108-5114

[42] Authentication of canned tuna and bonito by sequence and restriction site analysis of polymerase chain reaction products of mitochondrial DNA [J].

Ram, JL ;

Ram, ML ;

Baidoun, FF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (08) :2460-2467

[43] DNA-Based methods for the identification of commercial fish and seafood species [J].

Rasmussen, Rosalee S. ;

Morrissey, Michael T. .

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2008, 7 (03) :280-295

[44] Development of a method for genetic identification of four species of anchovies:: E-encrasicolus, E-anchoita, E-ringens and E-japonicus [J].

Santaclara, Francisco J. ;

Cabado, Ana G. ;

Vieites, Juan M. .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2006, 223 (05) :609-614

[45]

Scoles DR, 1998, FISH B-NOAA, V96, P823

[46] A simple method to distinguish two commercially valuable eel species in Japan Anguilla japonica and A-anguilla using polymerase chain reaction strategy with a species-specific primer [J].

Sezaki, K ;

Itoi, S ;

Watabe, S .

FISHERIES SCIENCE, 2005, 71 (02) :414-421

[47] Molecular identification methods of fish species: reassessment and possible applications [J].

Teletchea, Fabrice .

REVIEWS IN FISH BIOLOGY AND FISHERIES, 2009, 19 (03) :265-293

[48] Statistical validation of the identification of tuna species:: Bootstrap analysis of mitochondrial DNA sequences [J].

Terol, J ;

Mascarell, R ;

Fernandez-Pedrosa, V ;

Pérez-Alonso, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (05) :963-969

[49] Multiplex PCR method for use in real-time PCR for identification of fish fillets from grouper (Epinephelus and Mycteroperca species) and common substitute species [J].

Trotta, M ;

Schönhuth, S ;

Pepe, T ;

Cortesi, ML ;

Puyet, A ;

Bautista, JM .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (06) :2039-2045

[50] The complete mitochondrial genome of red grouper Plectropomus leopardus and its applications in identification of grouper species [J].

Zhu, Ze Yuan ;

Yue, Gen Hua .

AQUACULTURE, 2008, 276 (1-4) :44-49

← 1 2 3 4 5 →