A multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed fish products

被引：33

作者：

Catanese, Gaetano ^{[1
]}

Manchado, Manuel ^{[1
]}

Fernandez-Trujillo, Alejandra ^{[2
]}

Infante, Carlos ^{[1
]}

机构：

[1] IFAPA Ctr El Toruno, Lab Identificac Especies Pesqueras & Acuicolas, Cadiz 11500, Spain

[2] Univ Malaga, Fac Ciencias, Dept Microbiol, E-29071 Malaga, Spain

来源：

FOOD CHEMISTRY | 2010年 / 122卷 / 01期

关键词：

Scomber; Mackerels; Control region; Multiplex-PCR; Processed food; Authentication; RESTRICTION SITE ANALYSIS; DNA CONTROL REGION; SPECIES IDENTIFICATION; GROUPER EPINEPHELUS; GENETIC IDENTIFICATION; COMMERCIAL FISH; RFLP ANALYSIS; CANNED TUNA; POLYMORPHISM; BONITO;

D O I：

10.1016/j.foodchem.2010.02.036

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, we have developed a novel multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed food. The method consists of two novel Scomber japonicus- (104 bp) and Scomber australasicus-specific (143 bp) amplicons, respectively, corresponding to the mitochondrial control region. It also includes the previously described Scomber colias-specific product (159 bp) corresponding to the 5S ribosomal DNA, the Scomber scombrus-specific fragment (123 bp) from the mitochondrial NADH dehydrogenase subunit 5, and finally a positive amplification control corresponding to the small 12S rRNA subunit (188 bp). The system was assayed in fresh samples as well as in a total of 40 commercial samples including 28 different canned products and 12 unprocessed fresh fillets. A positive identification was observed in all cases according to their commercial labelling. Overall, this methodology reveals as a potential molecular tool for direct application in the authentication of Scomber mackerels in the seafood industry. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：319 / 326

页数：8

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