Identifying the Genes Responsible for Iron-Limited Condition in Riemerella anatipestifer CH-1 through RNA-Seq-Based Analysis

被引：17

作者：

Liu, MaFeng ^{[1
,2
,3
]}

Huang, Mi ^{[1
,2
,3
]}

Zhu, DeKang ^{[2
,3
]}

Wang, MingShu ^{[1
,2
,3
]}

Jia, RenYong ^{[1
,2
,3
]}

Chen, Shun ^{[1
,2
,3
]}

Sun, KunFeng ^{[1
,3
]}

Yang, Qiao ^{[1
,2
,3
]}

Wu, Ying ^{[1
,2
,3
]}

Biville, Francis ^{[4
]}

Cheng, AnChun ^{[1
,2
,3
]}

机构：

[1] Sichuan Agr Univ, Inst Prevent Vet Med, Chengdu 611130, Sichuan, Peoples R China

[2] Sichuan Agr Univ, Coll Vet Med, Res Ctr Avian Dis, Chengdu 611130, Sichuan, Peoples R China

[3] Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China

[4] Inst Pasteur, Dept Infect & Epidemiol, Unite Infect Bacteriennes Invas, Paris, France

来源：

BIOMED RESEARCH INTERNATIONAL | 2017年 / 2017卷

基金：

中国国家自然科学基金;

关键词：

DIFFERENTIAL EXPRESSION ANALYSIS; ESCHERICHIA-COLI; ACQUISITION; SYSTEM; IDENTIFICATION; MECHANISM; VIRULENCE; REVEALS; PROTEIN; TONB;

D O I：

10.1155/2017/8682057

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

One of the important elements for most bacterial growth is iron, the bioavailability of which is limited in hosts. Riemerella anatipestifer (R. anatipestifer, RA), an important duck pathogen, requires iron to live. However, the genes involved in iron metabolism and the mechanisms of iron transport are largely unknown. Here, we investigated the transcriptomic effects of iron limitation condition on R. anatipestifer CH-1 using the RNA-Seq and RNA-Seq-based analysis. Data analysis revealed genes encoding functions related to iron homeostasis, including a number of putative TonB-dependent receptor systems, a HmuY-like protein-dependent hemin (an iron-containing porphyrin) uptake system, a Feo system, a gene cluster related to starch utilization, and genes encoding hypothetical proteins that were significantly upregulated in response to iron limitation. Compared to the number of upregulated genes, more genes were significantly downregulated in response to iron limitation. The downregulated genes mainly encoded a number of outer membrane receptors, DNA-binding proteins, phage-related proteins, and many hypothetical proteins. This information suggested that RNA-Seq-based analysis in iron-limited medium is an effective and fast method for identifying genes involved in iron uptake in R. anatipestifer CH-1.

引用

页数：10