The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography

被引：18

作者：

Mathews, Irimpan I. ^{[1
]}

Allison, Kim ^{[1
]}

Robbins, Thomas ^{[2
]}

Lyubimov, Artem Y. ^{[1
,3
]}

Uervirojnangkoorn, Monarin ^{[3
]}

Brunger, Axel T. ^{[3
,4
]}

Khosla, Chaitan ^{[2
,5
,6
]}

DeMirci, Hasan ^{[1
,7
,8
]}

McPhillips, Scott E. ^{[1
]}

Hollenbeck, Michael ^{[1
]}

Soltis, Michael ^{[1
]}

Cohen, Aina E. ^{[1
]}

机构：

[1] Stanford Synchrotron Radiat Lightsource, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA

[2] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

[6] Stanford Univ, Stanford ChEM H, Stanford, CA 94305 USA

[7] SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA

[8] SLAC Natl Accelerator Lab, Biosci Div, Menlo Pk, CA 94025 USA

来源：

BIOCHEMISTRY | 2017年 / 56卷 / 36期

基金：

美国国家卫生研究院;

关键词：

BIOSYNTHESIS; MECHANISM;

D O I：

10.1021/acs.biochem.7b00711

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 angstrom using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of micro crystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed.

引用

页码：4751 / 4756

页数：6

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