Detection and Phylogenetic Analysis of Porcine Deltacoronavirus in Korean Swine Farms, 2015

被引:74
作者
Lee, J. H. [1 ,2 ]
Chung, H. C. [1 ,2 ]
Nguyen, V. G. [3 ]
Moon, H. J. [4 ]
Kim, H. K. [5 ]
Park, S. J. [6 ]
Lee, C. H. [1 ,2 ]
Lee, G. E. [1 ,2 ]
Park, B. K. [1 ,2 ]
机构
[1] Seoul Natl Univ, Dept Vet Med, Coll Vet Med, Virol Lab, DaeHakRo 1, Seoul 151742, South Korea
[2] Seoul Natl Univ, Res Inst Vet Sci, DaeHakRo 1, Seoul 151742, South Korea
[3] Vietnam Natl Univ Agr, Fac Vet Med, Dept Vet Microbiol & Infect Dis, Hanoi, Vietnam
[4] Green Cross Vet Prod, Res Unit, Yongin, South Korea
[5] Korea Res Inst Biosci & Biotechnol, Viral Infect Dis Res Ctr, Daejeon, South Korea
[6] Natl Forens Serv, Daegu Inst, Forens Med Div, Chilgok, South Korea
关键词
porcine deltacoronavirus; swine; South Korea; PIGS; PATHOGENICITY; DIARRHEA; STATES;
D O I
10.1111/tbed.12490
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
This study applied molecular-based method to investigate the presence of porcine deltacoronavirus (PDCoV) in 59 commercial pig farms in South Korea. The results of RT-PCR screening on a relatively large collection of faeces samples (n=681) from January 2013 to March 2015 did not reveal the presence of PDCoV until the end of 2014. However, on March 2015, PDCoV-positive samples (SL2, SL5) were detected from SL swine farm in Gyeongbuk province. The phylogenetic trees based on the complete spike- and nucleocapsid protein-coding genes showed that SL2 and SL5 closely related to the US PDCoV strains rather than those in China. Thought Korean strains of PDCoV isolated in 2014 (KNU14.04) and in 2015 (SL2 and SL5) grouped within US PDCoV cluster, the reconstruction of ancestral amino acid changes suggested that they are different.
引用
收藏
页码:248 / 252
页数:5
相关论文
共 17 条
[1]   Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets [J].
Chen, Qi ;
Gauger, Phillip ;
Stafne, Molly ;
Thomas, Joseph ;
Arruda, Paulo ;
Burrough, Eric ;
Madson, Darin ;
Brodie, Joseph ;
Magstadt, Drew ;
Derscheid, Rachel ;
Welch, Michael ;
Zhang, Jianqiang .
VIROLOGY, 2015, 482 :51-59
[2]   Porcine Deltacoronavirus in Mainland China [J].
Dong, Nan ;
Fang, Liurong ;
Zeng, Songlin ;
Sun, Qianqian ;
Chen, Huanchun ;
Xiao, Shaobo .
EMERGING INFECTIOUS DISEASES, 2015, 21 (12) :2254-2255
[3]   Isolation and Characterization of Porcine Deltacoronavirus from Pigs with Diarrhea in the United States [J].
Hu, Hui ;
Jung, Kwonil ;
Vlasova, Anastasia N. ;
Chepngeno, Juliet ;
Lu, Zhongyan ;
Wang, Qiuhong ;
Saif, Linda J. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (05) :1537-1548
[4]   Pathogenicity of 2 Porcine Deltacoronavirus Strains in Gnotobiotic Pigs [J].
Jung, Kwonil ;
Hu, Hui ;
Eyerly, Bryan ;
Lu, Zhongyan ;
Chepngeno, Juliet ;
Saif, Linda J. .
EMERGING INFECTIOUS DISEASES, 2015, 21 (04) :650-654
[5]   IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies [J].
Lam-Tung Nguyen ;
Schmidt, Heiko A. ;
von Haeseler, Arndt ;
Bui Quang Minh .
MOLECULAR BIOLOGY AND EVOLUTION, 2015, 32 (01) :268-274
[6]   Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South Korea [J].
Lee, Meong-Hun ;
Jeoung, Hye-Young ;
Park, Hye-Ran ;
Lim, Ji-Ae ;
Song, Jae-Young ;
An, Dong-Jun .
VIRUS GENES, 2013, 46 (01) :175-181
[7]   Complete Genome Characterization of Korean Porcine Deltacoronavirus Strain KOR/KNU14-04/2014 [J].
Lee, Sunhee ;
Lee, Changhee .
GENOME ANNOUNCEMENTS, 2014, 2 (06)
[8]   Origin, Evolution, and Virulence of Porcine Deltacoronaviruses in the United States [J].
Ma, Yuanmei ;
Zhang, Yu ;
Liang, Xueya ;
Lou, Fangfei ;
Oglesbee, Michael ;
Krakowka, Steven ;
Li, Jianrong .
MBIO, 2015, 6 (02)
[9]  
Marthaler D, 2014, EMERG INFECT DIS, V20, P1347, DOI [10.3201/eid2008.14-0526, 10.3201/eid2008.140526]
[10]   Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae [J].
Reuter, Gabor ;
Boldizsar, Akos ;
Pankovics, Peter .
ARCHIVES OF VIROLOGY, 2009, 154 (01) :101-108