Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2

被引:27
作者
Bonanomi, Dario
Rusconi, Laura
Colombo, Chiara Agnese
Benfenati, Fabio
Valtorta, Flavia
机构
[1] Vita Salute Univ, San Raffaele Sci Inst, I-20132 Milan, Italy
[2] Italian Inst Technol, Dept Neurosci, Genoa, Italy
[3] Univ Genoa, Dept Expt Med, I-16132 Genoa, Italy
[4] Italian Inst Technol, Res Unit Mol Neurosci, I-20132 Milan, Italy
关键词
endocytosis; exocytosis; synaptobrevin; 2; synaptophysin I; trafficking; vesicle-associated membrane protein 2 (VAMP2);
D O I
10.1042/BJ20061907
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Biogenesis and recycling of synaptic vesicles are accompanied by sorting processes that preserve the molecular composition of the compartments involved. In the present study, we have addressed the targeting of synaptobrevin 2/VAMP2 (vesicle-associated membrane protein 2), a critical component of the synaptic vesicle-fusion machinery, in a heterotypic context where its sorting is not confounded by the presence of other neuron-specific molecules. Ectopically expressed synaptophysin I interacts with VAMP2 and alters its default surface targeting to a prominent vesicular distribution, with no effect on the targeting of other membrane proteins. Protein-protein interaction is not sufficient for the control of VAMP2 sorting, which is mediated by the C-terminal domain of synaptophysin I. Synaptophysin I directs the sorting of VAMP2 to vesicles before surface delivery, without influencing VAMP2 endocytosis. Consistent with this, dynamin and a-SNAP(soluble N-ethylmaleimide-sensitive fusion protein-attachment protein) mutants which block trafficking at the plasma membrane do not abrogate the effect of synaptophysin I on VAMP2 sorting. These results indicate that the sorting determinants of synaptic vesicle proteins can operate independently of a neuronal context and implicate the association of VAMP2 with synaptophysin I in the specification of the pathway of synaptic vesicle biogenesis.
引用
收藏
页码:525 / 534
页数:10
相关论文
共 49 条
  • [1] Synaptic tetraspan vesicle membrane proteins are conserved but not needed for synaptogenesis and neuronal function in Caenorhabditis elegans
    Abraham, Christian
    Hutter, Harald
    Palfreyman, Mark T.
    Spatkowski, Gabriele
    Weimer, Robby M.
    Windoffer, Reinhard
    Jorgensen, Erik M.
    Leube, Rudolf E.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (21) : 8227 - 8232
  • [2] Assembly of presynaptic active zones from cytoplasmic transport packets
    Ahmari, SE
    Buchanan, J
    Smith, SJ
    [J]. NATURE NEUROSCIENCE, 2000, 3 (05) : 445 - 451
  • [3] Chronic blockade of glutamate receptors enhances presynaptic release and downregulates the interaction between synaptophysin-synaptobrevin-vesicle-associated membrane protein 2
    Bacci, A
    Coco, S
    Pravettoni, E
    Schenk, U
    Armano, S
    Frassoni, C
    Verderio, C
    De Camilli, P
    Matteoli, M
    [J]. JOURNAL OF NEUROSCIENCE, 2001, 21 (17) : 6588 - 6596
  • [4] Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis
    Barnard, RJO
    Morgan, A
    Burgoyne, RD
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 139 (04) : 875 - 883
  • [5] BARNEKOW A, 1990, ONCOGENE, V5, P1019
  • [6] Taipoxin induces synaptic vesicle exocytosis and disrupts the interaction of synaptophysin I with VAMP2
    Bonanomi, D
    Pennuto, M
    Rigoni, M
    Rossetto, O
    Montecucco, C
    Valtorta, F
    [J]. MOLECULAR PHARMACOLOGY, 2005, 67 (06) : 1901 - 1908
  • [7] Protein sorting in the synaptic vesicle life cycle
    Bonanomi, Dario
    Benfenati, Fabio
    Valtorta, Flavia
    [J]. PROGRESS IN NEUROBIOLOGY, 2006, 80 (04) : 177 - 217
  • [8] CALAKOS N, 1994, J BIOL CHEM, V269, P24534
  • [9] COLOCALIZATION OF SYNAPTOPHYSIN WITH TRANSFERRIN RECEPTORS - IMPLICATIONS FOR SYNAPTIC VESICLE BIOGENESIS
    CAMERON, PL
    SUDHOF, TC
    JAHN, R
    DECAMILLI, P
    [J]. JOURNAL OF CELL BIOLOGY, 1991, 115 (01) : 151 - 164
  • [10] Ca2+-dependent formation of a dynamin-synaptophysin complex -: Potential role in synaptic vesicle endocytosis
    Daly, C
    Ziff, EB
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (11) : 9010 - 9015