Method for determination of fatty acids in bovine colostrum using GC-FID

被引：23
作者：
Yurchenko, S. [1 ]
Sats, A. [1 ]
Poikalainen, V. [1 ]
Karus, A. [1 ]
机构：
[1] Estonian Univ Life Sci, Inst Vet Med & Anim Sci, Dept Food Sci & Technol, Kreutzwaldi 62, EE-51014 Tartu, Estonia
来源：
FOOD CHEMISTRY | 2016年 / 212卷
关键词：
Fatty acid methyl ester; Bovine colostrum; Boron trifluoride; Derivatization; Nitroterephthalic acid modified polyethyleneglycol; Holstein-Friesian cattle; PERFORMANCE LIQUID-CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; DAIRY-COWS; ESTERS; MILK; QUANTIFICATION; SPECTROSCOPY; VALIDATION; ISOMERS;
D O I：
10.1016/j.foodchem.2016.05.103
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Bovine colostrum is potentially valuable source of essential fatty acids (FAs), but so far only few studies have made the effort to estimate FA composition of this potential resource. The aim of current research was to fill this gap with selecting and validating an accurate procedure for the analysis of the composition of the FAs in bovine colostrum. We used colostrum samples of Holstein-Friesian cattle from Marja experimental farm as a test material. The validated method includes derivatization, in which FAs are sent through esterification with the acidic catalyst boron trifluoride. Formed methyl esters of fatty acids (FAMEs) were analysed using GC-FID. The obtained LOD and the LOQ of FAMEs were 0.11-0.68 and 0.37-2.27 ppm, respectively. The analysis of fortified samples showed very good and similar recoveries, indicating that the method proposed here can be routinely used for determination and investigation of the fatty acids in dairy products. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：117 / 122
页数：6
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