Reversal of multidrug resistance of gastric cancer cells by down-regulation of ZNRD1 with ZNRD1 siRNA
被引:28
作者:
Hong, L
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Hong, L
[1
]
Ning, X
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Ning, X
[1
]
Shi, Y
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Shi, Y
[1
]
Shen, H
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Shen, H
[1
]
Zhang, Y
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Zhang, Y
[1
]
Lan, M
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Lan, M
[1
]
Liang, S
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Liang, S
[1
]
Wang, J
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Wang, J
[1
]
Fan, D
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Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
Fan, D
[1
]
机构:
[1] Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian 710032, Peoples R China
drug resistance;
neoplasm;
genes;
MDR;
ZNRD;
stomach neoplasms;
D O I:
10.1080/09674845.2004.11732673
中图分类号:
R446 [实验室诊断];
R-33 [实验医学、医学实验];
学科分类号:
1001 ;
摘要:
The over-expression of a new zinc ribbon (ZNRD1) gene has been shown previously to promote a multidrug-resistant phenotype in gastric cancer cells through the up-regulation of permeability-glycoprotein (P-gp). In the present study, siRNA eukaryotic expression vectors of ZNRD1 are constructed and transfected into SGC7901/VCR cells to examine whether or not down-regulation of ZNRD1 increases cell sensitivity towards chemotherapeutic drugs. After transfection, ZNRD1 expression decreased dramatically in ZNRD1 siRNA transfectants compared with that in parental cells and empty vector control cells. Down-regulation of ZNRD1 significantly enhanced the sensitivity of SGC7901/VCR cells to vincristine, adriamycin and etoposide, but not to 5-fluorouracil and cisplatin. Cell capacity to efflux adriamycin decreased markedly in ZNRD1 siRNA transfectants, and correlation between ZNRD1 down-regulation and increased multidrug resistance 1 (MDR1) gene transcriptional activity was observed. These results suggest that the ZNRD1 siRNA constructs down-regulate the expression of ZNRD1 effectively and reverse the resistant phenotype of gastric cancer cells. Furthermore, ZNRD1 might influence transcription of the MDR1 gene and thus play an important role in multidrug resistance in gastric carcinoma.
机构:
Univ Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, England
Cabart, P
;
Murphy, S
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Univ Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, England
机构:
Univ Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, England
Cabart, P
;
Murphy, S
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机构:
Univ Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, England