DEDD negatively regulates transforming growth factor-β1 signaling by interacting with Smad3

被引：8
作者：
Xue, Jian-Fei
Hua, Fang
Lv, Qi
Lin, Heng
Wang, Zi-Yan
Yan, Jun
Liu, Jin-Wen
Lv, Xiao-Xi
Yang, Hong-Zhen
Hu, Zhuo-Wei [1 ]
机构：
[1] Chinese Acad Med Sci, Inst Mat Med, Mol Immunol & Pharmacol Lab, Beijing 100050, Peoples R China
来源：
FEBS LETTERS | 2010年 / 584卷 / 14期
基金：
中国国家自然科学基金;
关键词：
DEDD; Smad3; TGF-beta; 1; Signal transduction; Cancer; Invasion; DEATH-EFFECTOR DOMAIN; TGF-BETA; APOPTOSIS; SENSITIVITY; INHIBITION; FILAMENTS; CANCER; ROLES;
D O I：
10.1016/j.febslet.2010.05.043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transforming growth factor-beta 1 (TGF-beta 1) regulates a wide variety of cellular responses, such as proliferation, differentiation, migration and apoptosis. Here we report that death effector domain-containing DNA-binding protein (DEDD) physically interacts with Smad3. The inhibition of Smad3 by DEDD resulted in a reduction in TGF-beta 1/Smad3-mediated transcription. DEDD inhibited the functions of Smad3 by preventing Smad3 phosphorylation, which led to the reduced expression of TGF-beta 1/Smad3-targeted genes. TGF-beta 1 inhibited DEDD expression, and DEDD inhibited TGF-beta 1-mediated invasion. Therefore, our findings suggest that through its interaction with Smad3, DEDD is a novel negative regulator of the TGF-beta 1 signaling pathway. Structured summary: MINT-7895480: DEDD (uniprotkb:O75618) physically interacts (MI:0915) with Smad3 (uniprotkb:P84022) by anti bait co-immunoprecipitation (MI:0006) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3028 / 3034
页数：7
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