DNA binding and aggregation by carbon nanoparticles

被引：44

作者：

An, Hongjie ^{[1
,2
]}

Liu, Qingdai ^{[2
]}

Ji, Qiaoli ^{[2
]}

Jin, Bo ^{[1
]}

机构：

[1] Univ Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia

[2] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Bio X Ctr, Tianjin 300457, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 393卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Carbon nanoparticles (CNP); DNA; Binding; Aggregation; Atomic force microscopy (AFM); ATOMIC-FORCE MICROSCOPY; GOLD NANOPARTICLES; LASER PYROLYSIS; AIR-POLLUTION; STRANDED-DNA; NANOTUBES; TOXICITY; NANOTOXICOLOGY; NANOPOWDERS; CELLS;

D O I：

10.1016/j.bbrc.2010.02.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Significant environmental and health risks due to the increasing applications of engineered nanoparticles in medical and industrial activities have been concerned by many communities. The interactions between nanomaterials and genomes have been poorly studied so far. This study examined interactions of DNA with carbon nanoparticles (CNP) using atomic force microscopy (AFM). We experimentally assessed how CNP affect DNA molecule and bacterial growth of Escherichia coli. We found that CNP were bound to the DNA molecules during the DNA replication in vivo. The results revealed that the interaction of DNA with CNP resulted in DNA molecule binding and aggregation both in vivo and in vitro in a dose-dependent manner, and consequently inhabiting the E. coli growth. While this was a preliminary study, our results showed that this nanoparticle may have a significant impact on genomic activities. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：571 / 576

页数：6

共 35 条

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