PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies

被引：11

作者：

Zhou, JF ^{[1
]}

Wang, ZW ^{[1
]}

Ye, YZ ^{[1
]}

Wu, QJ ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2003年 / 48卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Cyprinus carpio; subspecies; ND5/6; PCR-RFLP; molecular marker;

D O I：

10.1360/03tb9098

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cyprinus carpio haematopterus and Cyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently. The results indicated that each subspecies owned one hyplotype and four restriction enzymes (Dde I, HaeIII, Taq I and Mbo I) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp.

引用

页码：465 / 468

页数：4

共 15 条

[1]

Avise John C., 1994, pi

[2] ORIGIN AND DOMESTICATION OF THE WILD CARP, CYPRINUS-CARPIO - FROM ROMAN GOURMETS TO THE SWIMMING FLOWERS [J].