Genomic sequence analysis and biological characteristics of a rescued clone of avian leukosis virus strain JS']JS11C1, isolated from indigenous chickens

被引：56

作者：

Cui, Ning ^{[1
,2
]}

Su, Shuai ^{[1
,2
]}

Chen, Zimeng ^{[1
,2
]}

Zhao, Xiaomin ^{[1
,2
]}

Cui, Zhizhong ^{[1
,2
]}

机构：

[1] Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China

[2] Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

来源：

JOURNAL OF GENERAL VIROLOGY | 2014年 / 95卷

基金：

中国国家自然科学基金;

关键词：

LONG TERMINAL REPEAT; GLIOMA-INDUCING VIRUS; CARDIAC ACTIN GENE; FOWL GLIOMA; SUBGROUP; IDENTIFICATION; INFECTION; BINDING; CARG; TRANSCRIPTION;

D O I：

10.1099/vir.0.067264-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The strain JS11C1, a member of a putative new subgroup of avian leukosis virus (ALV) that is different from all six known subgroups from chickens based on Gp85 amino acid sequence comparison, was isolated from Chinese native chicken breeds in 2012. In order to further study the genome structure, biological characteristics, and the evolutionary relationship of the virus with others of known subgroups from infected chickens, we determined the complete genome sequence, constructed an infectious clone of ALV strain JS11C1, and performed comparative analysis using the whole genome sequence or elements with that of other ALVs available in GenBank. The results showed that the full-length sequence of the JS11C1 DNA provirus genome was 7707 bp, which is consistent with a genetic organization typical of a replication-competent type C retrovirus lacking viral oncogenes. The rescued infectious clone of JS11C1 showed similar growth rate and biological characteristics to its original virus. All the comparison analyses based on whole genomes support the opinion that the new isolates are relatively distantly related to any known subgroups of ALVs and might be classified as a new subgroup.

引用

页码：2512 / 2522

页数：11

共 33 条

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