Improved propionic acid production from glycerol: Combining cyclic batch- and sequential batch fermentations with optimal nutrient composition

被引：32

作者：

Dishisha, Tarek ^{[1
]}

Ibrahim, Mohammad H. A. ^{[1
]}

Cavero, Victor Hugo ^{[1
]}

Alvarez, Maria Teresa ^{[1
]}

Hatti-Kaul, Rajni ^{[1
]}

机构：

[1] Lund Univ, Ctr Chem & Chem Engn, Dept Biotechnol, SE-22100 Lund, Sweden

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 176卷

关键词：

Propionic acid; Propionibacterium acidipropionici; Carbon:nitrogen ratio; Cyclic batch fermentation; Sequential batch fermentation; PROPIONIBACTERIUM-ACIDIPROPIONICI; IMMOBILIZED CELLS; TOLERANT STRAIN; WHEY PERMEATE; BACTERIA; GROWTH;

D O I：

10.1016/j.biortech.2014.11.013

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (w w(-1)) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93 mol% and productivity of 0.53 g L-1 h(-1). Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1 g L-1 h(-1) at initial glycerol up to 120 g L-1, and a maximum of 1.63 g L-1 h(-1) from 90 g L-1 glycerol. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：80 / 87

页数：8

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