PPG Peptide Nucleic Acids that Promote DNA Guanine Quadruplexes

被引：6

作者：

Englund, Ethan A. ^{[1
]}

Gupta, Pankaj ^{[1
]}

Micklitsch, Christopher M. ^{[1
]}

Onyshchenko, Mykola I. ^{[2
]}

Remeeva, Evgenia ^{[2
]}

Neumann, Ronald D. ^{[2
]}

Panyutin, Igor G. ^{[2
]}

Appella, Daniel H. ^{[1
]}

机构：

[1] NIDDK, Bioorgan Chem Lab, NIH, DHHS, Bethesda, MD 20892 USA

[2] NIH, Ctr Clin, DHHS, Bethesda, MD 20892 USA

来源：

CHEMBIOCHEM | 2014年 / 15卷 / 13期

基金：

美国国家卫生研究院;

关键词：

DNA recognition; G-quadruplexes; nucleic acids; nucleobases; peptide nucleic acids; DUPLEX DNA; STRAND INVASION; HUMAN GENOME; PNA; SEQUENCE; OLIGODEOXYRIBONUCLEOTIDES; STABILIZATION; SPECIFICITY; INHIBITORS; STABILITY;

D O I：

10.1002/cbic.201402224

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have shown that guanine-rich (G-rich) sequences with the potential to form quadruplexes might play a role in normal transcription as well as overexpression of oncogenes. Chemical tools that allow examination of the specific roles of G-quadruplex formation in vivo, and their association with gene regulation will be essential to understanding the functions of these quadruplexes and might lead to beneficial therapies. Properly designed peptide nucleic acids (PNAs) can invade G-rich DNA duplexes and induce the formation of a G-quadruplex in the free DNA strand. Replacing guanines in the PNA sequence with pyrazolo[3,4-d]pyrimidine guanine (PPG) nucleobases eliminates G-quadruplex formation with PNA and promotes invasion of the target DNA.

引用

页码：1887 / 1890

页数：4

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