Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

被引：14

作者：

Pryadko, Artyom S. A. ^{[1
]}

Mukhortova, Yulia R. ^{[1
]}

Chernozem, Roman V. ^{[1
]}

Pariy, Igor ^{[1
]}

Alipkina, Svetlana I. ^{[2
]}

Zharkova, Irina I. ^{[2
]}

Dudun, Andrey A. ^{[3
]}

Zhuikov, Vsevolod A. ^{[3
]}

Moisenovich, Anastasia M. ^{[2
]}

Bonartseva, Garina A. ^{[3
]}

Voinova, Vera V. ^{[2
]}

Chesnokova, Dariana V. ^{[2
]}

Ivanov, Alexey A. ^{[1
]}

Travnikova, Daria Yu. ^{[2
]}

Shaitan, Konstantin V. ^{[2
]}

Bonartsev, Anton P. ^{[2
]}

Wagner, Dmitry V. ^{[4
]}

Shlapakova, Lada E. ^{[1
]}

Surmenev, Roman A. ^{[1
]}

Surmeneva, Maria A. ^{[1
]}

机构：

[1] Natl Res Tomsk Polytech Univ, Phys Mat Sci & Composite Mat Ctr, Res Sch Chemistr y & Appl Biomed Sci, Tomsk 634050, Russia

[2] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119234, Russia

[3] Russian Acad Sci, Res Ctr Biotechnol, Moscow 119071, Russia

[4] Natl Res Tomsk State Univ, Tomsk 634050, Russia

来源：

ACS APPLIED BIO MATERIALS | 2022年 / 5卷 / 08期

基金：

俄罗斯科学基金会;

关键词：

magnetite; composite scaffold; poly-3-hydroxybutyrate; electrospinning; magnetization; IN-VITRO; MECHANICAL-PROPERTIES; ENZYMATIC DEGRADATION; BIOACTIVE GLASS; NANOPARTICLES; ACID); LIPASE; CRYSTALLIZATION; FABRICATION; MORPHOLOGY;

D O I：

10.1021/acsabm.2c00496

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Magnetically responsive composite polymer scaffolds have good potential for a variety of biomedical applications. In this work, electrospun composite scaffolds made of polyhydroxybutyrate (PHB) and magnetite (Fe3O4) particles (MPs) were studied before and after degradation in either PBS or a lipase solution. MPs of different sizes with high saturation magnetization were synthesized by the coprecipitation method followed by coating with citric acid (CA). Nanosized MPs were prone to magnetite-maghemite phase transformation during scaffold fabrication, as revealed by Raman spectroscopy; however, for CA-functionalized nanoparticles, the main phase was found to be magnetite, with some traces of maghemite. Submicron MPs were resistant to the magnetite-maghemite phase transformation. MPs did not significantly affect the morphology and diameter of PHB fibers. The scaffolds containing CA-coated MPs lost 0.3 or 0.2% of mass in the lipase solution and PBS, respectively, whereas scaffolds doped with unmodified MPs showed no mass changes after 1 month of incubation in either medium. In all electrospun scaffolds, no alterations of the fiber morphology were observed. Possible mechanisms of the crystalline-lamellar-structure changes in hybrid PHB/Fe3O4 scaffolds during hydrolytic and enzymatic degradation are proposed. It was revealed that particle size and particle surface functionalization affect the mechanical properties of the hybrid scaffolds. The addition of unmodified MPs increased scaffolds' ultimate strength but reduced elongation at break after the biodegradation, whereas simultaneous increases in both parameters were observed for composite scaffolds doped with CA-coated MPs. The highest saturation magnetization-higher than that published in the literature-was registered for composite PHB scaffolds doped with submicron MPs. All PHB scaffolds proved to be biocompatible, and the ones doped with nanosized MPs yielded faster proliferation of rat mesenchymal stem cells. In addition, all electrospun scaffolds were able to support angiogenesis in vivo at 30 days after implantation in Wistar rats.

引用

页码：3999 / 4019

页数：21

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