Six new tigliane diterpenoids with anti-inflammatory activity from Euphorbia kansuensis

Six new tigliane diterpenoids, euphkanoids A -F (1-6), along with 12 known diterpenoids (7-18) were isolated from the roots of Euphorbia kansuensis, a traditional Chinese medicinal plant. Their structures were elucidated by spectroscopic analysis, ECD calculation and chemical methods. Compounds 1-3 represent the unusual examples of tigliane diterpenoids with a conjugated olefinic aldehyde moiety. 1-18 and two hydrolyzed analogues (1c and 4a) were screened for the inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW264.7 cells. The results showed that 1-3 and 6 markedly inhibited NO production with IC(50 )values in the range of 4.8-11.3 lM, which were more active than the positive control quercetin (IC50 = 12.3 lM). (C) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.