Phytochemical studies and antibacterial activity of the aerial parts of physospermum verticillatum

In this study, we focused on the isolation and identification of the main compounds, triterpene, sterol, phenolic and fatty acid from the aerial parts of Physospermum verticillatum, which has not been previously reported. Dichloromethane, ethyl acetate and n-butanol extracts led to the isolation of beta-amyrin (1), 3,11-dihydroxy-12-oleanene (2), pentadecanoic acid (3), spina-sterol (4), spinasterol-3-O-beta-D-glucopyranoside (5) and saikochromic acid (6). These compounds were determined by means of the combined systems with high resolution tandem mass spectrometry (HPLC-TOF/MS), 1D NMR (H-1 NMR, C-13 NMR) and 2D NMR(COSY, HSQC and HMBC). Further, the extracts demonstrated significant antibacterial activity.