Carbon metabolism-mediated myogenic differentiation

被引：74
作者：
Bracha, Abigail L. [2 ,3 ,4 ]
Ramanathan, Arvind [1 ]
Huang, Sui [5 ]
Ingber, Donald E. [2 ,3 ,4 ,6 ,7 ]
Schreiber, Stuart L. [1 ,8 ]
机构：
[1] Broad Inst Harvard & MIT, Howard Hughes Med Inst, Cambridge, MA USA
[2] Childrens Hosp, Dept Pathol, Vasc Biol Program, Boston, MA 02115 USA
[3] Childrens Hosp, Dept Surg, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Boston, MA USA
[5] Univ Calgary, Inst Biocomplex & Informat, Calgary, AB, Canada
[6] Harvard Univ, Wyss Inst Biologically Inspired Engn, Boston, MA 02115 USA
[7] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[8] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
来源：
NATURE CHEMICAL BIOLOGY | 2010年 / 6卷 / 03期
基金：
美国国家科学基金会;
关键词：
HEXOSE-6-PHOSPHATE DEHYDROGENASE; STRESS;
D O I：
10.1038/NCHEMBIO.301
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The role of nutrients and metabolism in cellular differentiation is poorly understood. Using RNAi screening, metabolic profiling and small-molecule probes, we discovered that the knockdown of three metabolic enzymes-phosphoglycerate kinase (Pgk1), hexose-6-phosphate dehydrogenase (H6pd) and ATP citrate lyase (Acl)-induces differentiation of mouse C2C12 myoblasts even in the presence of mitogens. These enzymes and the pathways they regulate provide new targets for the control of myogenic differentiation in myoblasts and rhabdomyosarcoma cells.
引用
收藏
页码：202 / 204
页数：3
相关论文
共 16 条
  • [1] BARASH V, 1990, BIOCHEM INT, V20, P267
  • [2] ATP-citrate lyase deficiency in the mouse
    Beigneux, AP
    Kosinski, C
    Gavino, B
    Horton, JD
    Skarnes, WC
    Young, SG
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (10) : 9557 - 9564
  • [3] Modeling DNA sequence-based cis-regulatory gene networks
    Bolouri, H
    Davidson, EH
    [J]. DEVELOPMENTAL BIOLOGY, 2002, 246 (01) : 2 - 13
  • [4] Dagher R, 1999, Oncologist, V4, P34
  • [5] ATP citrate lyase inhibition can suppress tumor cell growth
    Hatzivassiliou, G
    Zhao, FP
    Bauer, DE
    Andreadis, C
    Shaw, AN
    Dhanak, D
    Hingorani, SR
    Tuveson, DA
    Thompson, CB
    [J]. CANCER CELL, 2005, 8 (04) : 311 - 321
  • [6] Minireview:: Hexose-6-phosphate dehydrogenase and redox control of 11β-hydroxysteroid dehydrogenase type 1 activity
    Hewitt, KN
    Walker, EA
    Stewart, PM
    [J]. ENDOCRINOLOGY, 2005, 146 (06) : 2539 - 2543
  • [7] Deletion of hexose-6-phosphate dehydrogenase activates the unfolded protein response pathway and induces skeletal myopathy
    Lavery, Gareth G.
    Walker, Elizabeth A.
    Turan, Nil
    Rogoff, Daniela
    Ryder, Jeffery W.
    Shelton, John M.
    Richardson, James A.
    Falciani, Francesco
    White, Perrin C.
    Stewart, Paul M.
    Parker, Keith L.
    McMillan, Daniel R.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (13) : 8453 - 8461
  • [8] Molecular framework underlying pluripotency
    Loh, Yuin-Han
    Ng, Jia-Hui
    Ng, Huck-Hui
    [J]. CELL CYCLE, 2008, 7 (07) : 885 - 891
  • [9] Endoplasmic reticulum stress increases myofiber formation in vitro
    Nakanishi, Keiko
    Dohmae, Naoshi
    Morishima, Nobuhiro
    [J]. FASEB JOURNAL, 2007, 21 (11) : 2994 - 3003
  • [10] Chemical chaperones reduce ER stress and restore glucose homeostasis in a mouse model of type 2 diabetes
    Oezcan, Umut
    Yilmaz, Erkan
    Oezcan, Lale
    Furuhashi, Masato
    Vaillancourt, Eric
    Smith, Ross O.
    Goerguen, Cem Z.
    Hotamisligil, Goekhan S.
    [J]. SCIENCE, 2006, 313 (5790) : 1137 - 1140
12