Assessment of Cd-induced genotoxic damage in Urtica pilulifera L. using RAPD-PCR analysis

被引:17
作者
Dogan, Ilhan [1 ,2 ]
Ozyigit, Ibrahim Ilker [3 ]
Tombuloglu, Guzin [4 ]
Sakcali, Mehmet Serdal [5 ]
Tombuloglu, Huseyin [6 ]
机构
[1] Izmir Inst Technol, Dept Mol Biol & Genet, Fac Sci, Izmir, Turkey
[2] Kyrgyz Turkish Manas Univ, Dept Biol, Fac Sci, Bishkek, Kyrgyzstan
[3] Marmara Univ, Dept Biol, Fac Arts & Sci, Istanbul, Turkey
[4] Fatih Univ, Vocat Sch Med Sci, Program Pathol Lab Tech, Istanbul, Turkey
[5] Suleyman Demirel Univ, Fac Arts & Sci, Dept Biol, East Campus Cunur, TR-32200 Isparta, Turkey
[6] Fatih Univ, Dept Biol, Fac Arts & Sci, Istanbul, Turkey
关键词
RAPD-PCR; genotoxicity; cadmium (Cd); Roman nettle; heavy metals; AMPLIFIED POLYMORPHIC DNA; HEAVY-METALS; CADMIUM ACCUMULATION; PHOTOSYNTHESIS; TOLERANCE; ALUMINUM; SATIVUM; TOBACCO; GENOME; GROWTH;
D O I
10.1080/13102818.2015.1115371
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Plants can be used as biological indicators in assessing the damage done by bioaccumulation of heavy metals and their negative impact on the environment. In the present research, Roman nettle (Urtica pilulifera L.) was employed as a bioindicator for cadmium (Cd) pollution. The comparisons between unexposed and exposed plant samples revealed inhibition of the root growth (similar to 25.96% and similar to 45.92% after treatment with 100 and 200 mu mol/L Cd concentrations, respectively), reduction in the total soluble protein quantities (similar to 53.92% and similar to 66.29% after treatment with 100 and 200 mu mol/L Cd concentrations, respectively) and a gradual genomic instability when the Cd concentrations were increased. The results indicated that alterations in randomly amplified polymorphic DNA (RAPD) profiles, following the Cd treatments, included normal band losses and emergence of new bands, when compared to the controls. Also, the obtained data from F1 plants, utilized for analysis of genotoxicity, revealed that DNA alterations, occurring in parent plants due to Cd pollution, were transmitted to the next generation.
引用
收藏
页码:284 / 291
页数:8
相关论文
共 56 条
  • [1] Aly A. A., 2012, Journal of Stress Physiology & Biochemistry, V8, P241
  • [2] Qualitative assessment of genotoxicity using random amplified polymorphic DNA:: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene
    Atienzar, FA
    Conradi, M
    Evenden, AJ
    Jha, AN
    Depledge, MH
    [J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1999, 18 (10) : 2275 - 2282
  • [3] The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review
    Atienzar, Franck A.
    Jha, Awadhesh N.
    [J]. MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2006, 613 (2-3) : 76 - 102
  • [4] Baytop T, 1999, THERAPY MED PLANTS T, P1
  • [5] First evidence of fish genotoxicity induced by heavy metals from landfill leachates: The advantage of using the RAPD-PCR technique
    Ben Salem, Zohra
    Capelli, Nicolas
    Grisey, Elise
    Baurand, Pierre-Emmanuel
    Ayadi, Habib
    Aleya, Lotfi
    [J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2014, 101 : 90 - 96
  • [6] Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review)
    Bertin, G.
    Averbeck, D.
    [J]. BIOCHIMIE, 2006, 88 (11) : 1549 - 1559
  • [7] USE OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS IN COMPARATIVE GENOME STUDIES
    BOWDITCH, BM
    ALBRIGHT, DG
    WILLIAMS, JGK
    BRAUN, MJ
    [J]. MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, 1993, 224 : 294 - 309
  • [8] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [9] Heavy metal tolerance of Silene vulgaris
    Bringezu, K
    Lichtenberger, O
    Leopold, I
    Neumann, D
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 1999, 154 (04) : 536 - 546
  • [10] Assessment of air pollution genotoxicity by RAPD in Evernia prunastri L. Ach. from around iron-steel factory in Karabuk, Turkey
    Cansaran-Duman, Demet
    Atakol, Orhan
    Aras, Sumer
    [J]. JOURNAL OF ENVIRONMENTAL SCIENCES, 2011, 23 (07) : 1171 - 1178