A dimerization "switch" in the internalization mechanism of a cell-penetrating peptide

被引：13
作者：
Moss, JA
Lillo, A
Kim, YS
Gao, CS
Ditzel, H
Janda, KD
机构：
[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA
[3] Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 02期
关键词：
D O I：
10.1021/ja0443171
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The internalization mechanism of a cell-penetrating peptide has been explored through combinatorial selection of a phage-displayed peptide dimer library, chemical synthesis, and biophysical characterization. Both energy-dependent and energy-independent modes for peptide uptake by the target mammalian cells were observed, suggesting a role for higher-order structure in modulating the action of this novel cell-penetrating peptide. Copyright © 2005 American Chemical Society.
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页码：538 / 539
页数：2
相关论文
共 36 条
[1]  
Adda CG, 2002, COMB CHEM HIGH T SCR, V5, P1
[2]   ANALYSIS OF ARGININE-RICH PEPTIDES FROM THE HIV TAT PROTEIN REVEALS UNUSUAL FEATURES OF RNA PROTEIN RECOGNITION [J].
CALNAN, BJ ;
BIANCALANA, S ;
HUDSON, D ;
FRANKEL, AD .
GENES & DEVELOPMENT, 1991, 5 (02) :201-210
[3]   Molecular specialization of breast vasculature: A breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature [J].
Essler, M ;
Ruoslahti, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2252-2257
[4]   Cellular delivery of impermeable effector molecules in the form of conjugates with peptides capable of mediating membrane translocation [J].
Fischer, PM ;
Krausz, E ;
Lane, DP .
BIOCONJUGATE CHEMISTRY, 2001, 12 (06) :825-841
[5]   CELLULAR UPTAKE OF THE TAT PROTEIN FROM HUMAN IMMUNODEFICIENCY VIRUS [J].
FRANKEL, AD ;
PABO, CO .
CELL, 1988, 55 (06) :1189-1193
[6]   Arginine-rich peptides - An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery [J].
Futaki, S ;
Suzuki, T ;
Ohashi, W ;
Yagami, T ;
Tanaka, S ;
Ueda, K ;
Sugiura, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (08) :5836-5840
[7]   Arginine-rich peptides: potential for intracellular delivery of macromolecules and the mystery of the translocation mechanisms [J].
Futaki, S .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2002, 245 (1-2) :1-7
[8]   Making artificial antibodies: A format for phage display of combinatorial heterodimeric arrays [J].
Gao, CS ;
Mao, SL ;
Lo, CHL ;
Wirsching, P ;
Lerner, RA ;
Janda, KD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6025-6030
[9]   A method for the generation of combinatorial antibody libraries using pIX phage display [J].
Gao, CS ;
Mao, SL ;
Kaufmann, G ;
Wirsching, P ;
Lerner, RA ;
Janda, KD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12612-12616
[10]   A cell-penetrating peptide from a novel pVII-pIX phage-displayed random peptide library [J].
Gao, CS ;
Mao, SL ;
Ditzel, HJ ;
Farnaes, L ;
Wirsching, P ;
Lerner, RA ;
Janda, KD .
BIOORGANIC & MEDICINAL CHEMISTRY, 2002, 10 (12) :4057-4065
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