Maternal liver docosahexaenoic acid (DHA) stores are increased via higher serum unesterified DHA uptake in pregnant long Evans rats

被引：15

作者：

Metherel, Adam H. ^{[1
]}

Kitson, Alex P. ^{[1
]}

Domenichiello, Anthony F. ^{[1
]}

Lacombe, R. J. Scott ^{[1
]}

Hopperton, Kathryn E. ^{[1
]}

Trepanier, Marc-Olivier ^{[1
]}

Alashmali, Shoug M. ^{[1
]}

Lin, Lin ^{[1
]}

Bazinet, Richard P. ^{[1
]}

机构：

[1] Univ Toronto, Fac Med, Dept Nutr Sci, 150 Coll St,Room 307,Fitzgerald Bldg, Toronto, ON M5S 3E2, Canada

来源：

JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 2017年 / 46卷

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

Pregnancy; Metabolism; Lipids; Liver; Omega-3 fatty acids; Kinetics; ALPHA-LINOLENIC ACID; POLYUNSATURATED FATTY-ACIDS; FISH-OIL SUPPLEMENTATION; BODY SYNTHESIS RATES; ACYL-COA SYNTHETASE; BRAIN PHOSPHOLIPIDS; EICOSAPENTAENOIC ACID; ACCRETION RATES; DEFICIENT DIET; N-3;

D O I：

10.1016/j.jnutbio.2017.05.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Maternal docosahexaenoic acid (DHA, 22:6n-3) supplies the developing fetus during pregnancy; however, the mechanisms are unclear. We utilized pregnant rats to determine rates of DHA accretion, tissue unesterified DHA uptake and whole-body DHA synthesis-secretion. Female rats maintained on a DHA-free, 2% alpha-linolenic acid diet were either:1) sacrificed at 56 days for baseline measures, 2) mated and sacrificed at 14-18 days of pregnancy or 3) or sacrificed at 14-18 days as age-matched virgin controls. Maternal brain, adipose, liver and whole body fatty acid concentrations was determined for balance analysis, and kinetic modeling was used to determine brain and liver plasma unesterified DHA uptake and whole-body DHA synthesis-secretion rates. Total liver DHA was significantly higher in pregnant (95 +/- 5 mu mol) versus non-pregnant (49 +/- 5) rats with no differences in whole-body DHA synthesis-secretion rates. However, liver uptake of plasma unesterified DHA was 3.8-fold higher in pregnant animals compared to non-pregnant controls, and periuterine adipose DHA was lower in pregnant (0.89 +/- 0.09 mu mol/g) versus non-pregnant (1.26 +/- 0.06) rats. In conclusion, higher liver DHA accretion during pregnancy appears to be driven by higher unesterified DHA uptake, potentially via DHA mobilization from periuterine adipose for delivery to the fetus during the brain growth spurt. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：143 / 150

页数：8

共 47 条

[1] MATERNAL ESSENTIAL FATTY-ACID PATTERNS DURING NORMAL-PREGNANCY AND THEIR RELATIONSHIP TO THE NEONATAL ESSENTIAL FATTY-ACID STATUS [J].