Cloning and expression of rat GFAP cDNA in Escherichia coli

被引：5
|
作者：
Chekhonin, V. P.
Pavlov, K. A.
Shkoporov, A. N. [1 ]
Yefimov, B. A.
Gurina, O. I.
Dmitrieva, T. B.
机构：
[1] VP Serbskii Ctr Social & Forens Psychiat, Dept Biol Psychiat, Lab Immunochem, Moscow, Russia
[2] Russian State Med Univ, Dept Basic & Clin Microbiol, Moscow 117437, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2007年 / 143卷 / 01期
关键词：
glial fibrillary acidic protein (GFAP); GFAP recombinant protein; escherichia coli; central nervous system;
D O I：
10.1007/s10517-007-0019-9
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.
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收藏
页码：68 / 71
页数：4
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