Towards Fully Automated Phototransfection

被引：0
|
作者：
Cappelleri, David J. [1 ]
Halasz, Adam [4 ]
Sul, Jai-Yoon [3 ]
Kim, Tae Kyung [3 ]
Eberwine, James [3 ]
Kumar, Vijay [2 ]
机构：
[1] Stevens Inst Technol, Dept Mech Engn, Multo Scale Robot & Automat Lab, Hoboken, NJ 07030 USA
[2] Univ Pennsylvania, Dept Mech Engn, Grasp Lab, Philadelphia, PA USA
[3] Univ Pennsylvania, Dept Pharm, PENN Genome Front Inst, Philadelphia, PA USA
[4] W Virginia Univ, Dept Mat, Morgantown, WV USA
来源：
2009 IEEE INTERNATIONAL CONFERENCE ON AUTOMATION SCIENCE AND ENGINEERING | 2009年
关键词：
D O I：
10.1109/COASE.2009.5234165
中图分类号：
TP [自动化技术、计算机技术];
学科分类号：
0812 ;
摘要：
Flexible automation technologies have been applied to the manual phototransfection procedure on fibroblast and astrocyte cells. We have designed and implemented a framework for increased throughput of the entire process. Integrated image processing, laser target position calculation, and stage movements show a throughput increase of > 23X over the current manual method while the potential for even greater throughput improvements (> 110X) is described. A software tool for automated single cell morphological measurements has also been constructed and shown to be able quantify changes in the cell before and after the process, successfully characterizing them, using metrics such as cell perimeter, area, major and minor axis length, and eccentricity values.
引用
收藏
页码：202 / +
页数：2
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