Sperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism

被引:7
作者
Rossi, LM
Pereira, LAV
de Santis, L
Pasqualotto, FF
Iaconelli, A
Ortiz, V
Borges, E
机构
[1] Univ Fed Sao Paulo, Med Sch Jundiai, Sao Paulo, SP, Brazil
[2] Univ Fed Sao Paulo, Fertil Ctr, Sao Paulo, SP, Brazil
[3] Univ Fed Sao Paulo, Univ Estadual Campinas, Sao Paulo, SP, Brazil
[4] Univ Fed Sao Paulo, Dept Urol, Sao Paulo, SP, Brazil
[5] Univ Caxias Sul, Dept Embriol, Caxias do Sul, RS, Brazil
关键词
aspiration; cryptorchidism; extraction; sperm; testicle;
D O I
10.1093/humrep/deh549
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: Our aim was to assess the suppression of spermatogenesis and sperm retrieval rate after testicular sperm extraction (TESE) or testicular sperm aspiration (TESA) in adult rats with surgically induced cryptorchidism. Methods: Adult rats were submitted to TESE and TESA procedures after 15 days of induced cryptorchidism. After spermatozoa retrieval, the testicles were extracted, weighed and a morphological analysis by conventional light microscopy was done. The numbers of spermatozoa retrieved in both TESA and TESE were rated and compared. Results: Histological analysis of the testicles revealed Sertoli cell-only syndrome in 60% of the testicles, and maturation arrest in the remaining cryptorchid testicles. Significant differences were seen in the number of spermatozoa retrieved (P < 0.05) between cryptorchidic and control rats. When sperm retrieval techniques were compared, no differences were detected in the number of spermatozoa obtained (P > 0.05). Conclusions: It seems that a 15 day period of cryptorchidism is enough to induce spermatogenesis disorders. No differences were detected in the number of spermatozoa retrieved in the right or left testicles, irrespective of the testicular pole. Furthermore, and even more importantly, no differences in the retrieval rate were seen between the two techniques.
引用
收藏
页码:443 / 447
页数:5
相关论文
共 40 条
[1]  
Agarwala S, 1996, PEDIATR SURG INT, V11, P266, DOI 10.1007/BF00178434
[2]   A comparison of the histopathological findings after open and percutaneous needle testicular biopsy in adult male rats [J].
Altay, B ;
Hekimgil, M ;
Kefi, A ;
Girgin, C ;
Çikili, N .
BJU INTERNATIONAL, 2000, 86 (09) :1084-1087
[3]   Prospective comparative study between microsurgical and conventional testicular sperm extraction in nonobstructive azoospermia: follow-up by serial ultrasound examinations [J].
Amer, M ;
Ateyah, A ;
Hany, R ;
Zohdy, W .
HUMAN REPRODUCTION, 2000, 15 (03) :653-656
[4]   Ultrasound-guided testicular sperm aspiration in azoospermic patients: A new sperm retrieval method for intracytoplasmic sperm injection [J].
Belenky, A ;
Avrech, OM ;
Bachar, GN ;
Zuckerman, Z ;
Ben Rafael, Z ;
Fisch, B ;
Cohen, M .
JOURNAL OF CLINICAL ULTRASOUND, 2001, 29 (06) :339-343
[5]   FERTILIZING ABILITY OF TESTICULAR SPERMATOZOA [J].
CRAFT, I ;
BENNETT, V ;
NICHOLSON, N .
LANCET, 1993, 342 (8875) :864-864
[6]   Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia [J].
De Croo, I ;
Van der Elst, J ;
Everaert, K ;
De Sutter, P ;
Dhont, M .
HUMAN REPRODUCTION, 2000, 15 (06) :1383-1388
[7]   PREGNANCIES AFTER TESTICULAR SPERM EXTRACTION AND INTRACYTOPLASMIC SPERM INJECTION IN NONOBSTRUCTIVE AZOOSPERMIA [J].
DEVROEY, P ;
LIU, J ;
NAGY, Z ;
GOOSSENS, A ;
TOURNAYE, H ;
CAMUS, M ;
VANSTEIRTEGHEM, A ;
SILBER, S .
HUMAN REPRODUCTION, 1995, 10 (06) :1457-1460
[8]   A prospective study of multiple needle biopsies versus a single open biopsy for testicular sperm extraction in men with non-obstructive azoospermia [J].
Ezeh, UIO ;
Moore, HDM ;
Cooke, ID .
HUMAN REPRODUCTION, 1998, 13 (11) :3075-3080
[9]   Establishment of predictive variables associated with testicular sperm retrieval in men with non-obstructive azoospermia [J].
Ezeh, UIO ;
Taub, NA ;
Moore, HDM ;
Cooke, ID .
HUMAN REPRODUCTION, 1999, 14 (04) :1005-1012
[10]   Testicular sperm retrieval by percutaneous fine needle sperm aspiration compared with testicular sperm extraction by open biopsy in men with non-obstructive azoospermia [J].
Friedler, S ;
Raziel, A ;
Strassburger, D ;
Soffer, Y ;
Komarovsky, D ;
RonEl, R .
HUMAN REPRODUCTION, 1997, 12 (07) :1488-1493