Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., a medicinal woody species

被引：0

作者：

Harta, Monica ^{[1
]}

Pamfil, Doru ^{[1
]}

Ardelean, Marin ^{[2
]}

Pedersen, Carsten ^{[3
]}

Pop, Rodica ^{[1
]}

Cordea, Mirela ^{[2
]}

机构：

[1] Univ Agr Sci & Vet Med Cluj Napoca, Dept Biotechnol, Cluj Napoca 400372, Romania

[2] Univ Agr Sci & Vet Med Cluj Napoca, Dept Genet & Plant Breeding, Cluj Napoca 400372, Romania

[3] KVL Royal Vet & Agr Univ, DK-1870 Frederiksberg, Denmark

来源：

ROMANIAN BIOTECHNOLOGICAL LETTERS | 2010年 / 15卷 / 02期

关键词：

Ginkgo biloba; DNA polymorphism; RAPD; AFLP; fingerprinting techniques;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

引用

页码：56 / 61

页数：6

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