Peroxidase activity of cytochrome c

被引：0
|
作者：
Kim, NH
Jeong, MS
Choi, SY
Kang, JH [1 ]
机构：
[1] Cheongju Univ, Dept Genet Engn, Cheongju 360764, South Korea
[2] Hallym Univ, Div Life Sci, Dept Genet Engn, Chunchon 200702, South Korea
来源：
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2004年 / 25卷 / 12期
关键词：
cytochrome c; peroxidase; radical scavenger; iron chelator;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The peroxidase activity of cytochrome c was studied by using a chromogen, 2,2'-azinobis-(2-ethylbenzthiazoline-6-sulfonate) (ABTS). Initial rate of ABTS oxidation formation was linear with respect to the concentration of cytochrome c between 2.5-10 muM and H2O2 between 0.1-0.5 mM. The optimal pH for the peroxidase activity of cytochrome c was 7.0-8.5. The peroxidase activity retained about 40% of the maximum activity when exposed at 60 degreesC for 10 min. The peroxidase activity showed a typical Michaelis-Menten kinetics for H2O2 which Km value was 29.6 mM. Radical scavengers inhibited the peroxidase activity of cytochrome c. The peroxidase activity was significantly inhibited by the low concentration of iron chelator, deferoxamine. The results suggested that the peroxidase activity was associated with iron in the heme of cytochrome c.
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页码：1889 / 1892
页数：4
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