Glial cell line-derived neurotrophic factor (GDNF) and its receptors in human ovaries from fetuses, girls, and women

被引：15
作者：
Farhi, Jacob [1 ,2 ]
Ao, Asangla [3 ,4 ]
Fisch, Benjamin [1 ,2 ]
Zhang, Xiao Yun [3 ]
Garor, Roni [1 ,2 ]
Abir, Ronit [1 ,2 ]
机构：
[1] Beilinson Med Ctr, Helen Schneider Hosp Women, Rabin Med Ctr, Infertil & IVF Unit, IL-49100 Petah Tiqwa, Israel
[2] Tel Aviv Univ, Sackler Fac Med, IL-69978 Tel Aviv, Israel
[3] Royal Victoria Hosp, Dept Obstet & Gynecol, Belfast, Antrim, North Ireland
[4] McGill Univ, Dept Human Genet, Montreal, PQ, Canada
来源：
FERTILITY AND STERILITY | 2010年 / 93卷 / 08期
关键词：
Primordial follicle activation; GDNF; GFR-alpha 1 and RET receptor units; immunohistochemistry; in situ hybridization; RT-PCR; TYROSINE KINASE; MESSENGER-RNA; IN-VITRO; RET; ISOFORMS; DISEASE; MICE; EXPRESSION; MATURATION; NEURTURIN;
D O I：
10.1016/j.fertnstert.2009.09.047
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: To investigate the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptors, GDNF alpha 1 (GFR-alpha 1) and tyrosine kinase receptor for rearranged during transfection (RET), in human ovaries at the protein and mRNA levels. Design: Samples were prepared for immunohistochemical staining for GDNF, GFR-alpha 1, and RET and in situ hybridization for the mRNA of GFR-alpha 1. The mRNA expression of two GDNF isoforms and two RET isoforms was investigated by reverse transcription polymerase chain reaction. Setting: Infertility unit at a university-affiliated tertiary medical center. Patient(s): Fifteen patients who underwent pregnancy terminations at 21-35 gestational weeks and 28 girls/women aged 5-39 years who underwent ovarian laparoscopies. Intervention(s): None. Main Outcome Measure(s): Laboratory analysis of human ovarian specimens. Result(s): The GDNF protein was detected in oocytes of all samples. Granulosa cells (GCs) stained for GDNF in a portion of fetal samples and in all samples from girls/women. The proteins for GFR-alpha 1 and RET were detected in both oocytes and GCs with weak staining for the long RET isoform. GFR-alpha 1 mRNA transcripts were detected in oocytes and GCs from all samples. The mRNA transcripts for the two GDNF isoforms and the two RET isoforms were detected in all fetal and adult specimens. Conclusion(s): The presence of the receptors of GDNF in the GCs of human primordial follicles suggests that GDNF may be involved in the regulation of primordial follicular activation. (Fertil Steril (R) 2010; 93: 2565-71. (C) 2010 by American Society for Reproductive Medicine.)
引用
收藏
页码：2565 / 2571
页数：7
相关论文
共 27 条
[1]  
Abir R, 2006, HISTOL HISTOPATHOL, V21, P887, DOI 10.14670/HH-21.887
[2]   Presence of NGF and its receptors in ovaries from human fetuses and adults [J].
Abir, R ;
Fisch, B ;
Jin, S ;
Barnnet, M ;
Ben-Haroush, A ;
Felz, C ;
Kessler-Icekson, G ;
Feldberg, D ;
Nitke, S ;
Ao, A .
MOLECULAR HUMAN REPRODUCTION, 2005, 11 (04) :229-236
[3]  
ABIR R, 2008, NERVE GROWTH FACTOR, pCH9
[4]   Human GFRA1:: Cloning, mapping, genomic structure, and evaluation as a candidate gene for Hirschsprung disease susceptibility [J].
Angrist, M ;
Jing, SQ ;
Bolk, S ;
Bentley, K ;
Nallasamy, S ;
Halushka, M ;
Fox, GM ;
Chakravarti, A .
GENOMICS, 1998, 48 (03) :354-362
[5]   Age-dependent bimodal GDNF regulation during ovarian tumorigenesis in follitropin receptor mutant mice [J].
Aravindakshan, J. ;
Chen, X. L. ;
Sairam, M. R. .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 351 (02) :507-513
[6]   Gene expression patterns for GDNF and its receptors in the human putamen affected by Parkinson's disease:: A real-time PCR study [J].
Backman, Cristina M. ;
Shan, Lufei ;
Zhang, Ya Jun ;
Hoffer, Barry J. ;
Leonard, Sherry ;
Troncoso, Juan C. ;
Vonsatel, Paul ;
Tomac, Andreas C. .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2006, 252 (1-2) :160-166
[7]  
Choi-Lundberg D L, 1995, Brain Res Dev Brain Res, V85, P80
[8]   Differential activities of the RET tyrosine kinase receptor isoforms during mammalian embryogenesis [J].
de Graaff, E ;
Srinivas, S ;
Kilkenny, C ;
D'Agati, V ;
Mankoo, BS ;
Costantini, F ;
Pachnis, V .
GENES & DEVELOPMENT, 2001, 15 (18) :2433-2444
[9]   RET as a diagnostic and therapeutic target in sporadic and hereditary endocrine tumors [J].
de Groot, Jan Willem B. ;
Links, Thera P. ;
Plukker, John T. M. ;
Lips, Cornelis J. M. ;
Hofstra, Robert M. W. .
ENDOCRINE REVIEWS, 2006, 27 (05) :535-560
[10]   Cloning of a novel murine isoform of the glial cell line-derived neurotrophic factor receptor [J].
Dey, BK ;
Wong, YW ;
Too, HP .
NEUROREPORT, 1998, 9 (01) :37-42
123