Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe

被引：60

作者：

Sakai, H

Procop, GW

Kobayashi, N

Togawa, D

Wilson, DA

Borden, L

Krebs, V

Bauer, TW

机构：

[1] Cleveland Clin Fdn, Dept Anat Pathol, Surg Pathol Sect, Cleveland, OH 44195 USA

[2] Cleveland Clin Fdn, Dept Clin Pathol, Clin Microbiol Sect, Cleveland, OH 44195 USA

[3] Cleveland Clin Fdn, Dept Orthopaed Surg, Cleveland, OH 44195 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 12期

关键词：

D O I：

10.1128/JCM.42.12.5739-5744.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A real-time PCR assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. One probe set detects the Staphylococcus genus, whereas the other probe set is specific for Staphylococcus aureus. One hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. This assay was 100% sensitive and 100% specific for the detection of the Staphylococcus genus and of S. aureus.

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页码：5739 / 5744

页数：6

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