The same well-characterized T cell epitope SIINFEKL expressed in the context of a cytoplasmic or secreted protein in BCG induces different CD8+ T cell responses

被引：9

作者：

Hulseberg, Paul D. ^{[1
]}

Zozulya, Alla ^{[1
]}

Chu, Hamlet H. ^{[1
]}

Triccas, James A. ^{[2
]}

Fabry, Zsuzsanna ^{[1
]}

Sandor, Matyas ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Pathol & Lab Sci, Madison, WI 53706 USA

[2] Univ Sydney, Centenary Inst Canc Med & Cell Biol, Sydney, NSW 2006, Australia

来源：

IMMUNOLOGY LETTERS | 2010年 / 130卷 / 1-2期

关键词：

Mycobacterium; CD8(+) T cell responses; Antigen location; CLASS-I PRESENTATION; MYCOBACTERIUM-TUBERCULOSIS INFECTION; DENDRITIC CELLS; CROSS-PRESENTATION; ANTIGEN PRESENTATION; EXOGENOUS ANTIGENS; LISTERIA-MONOCYTOGENES; PARTICULATE ANTIGENS; PROCESSING PATHWAY; TAP-DEFICIENT;

D O I：

10.1016/j.imlet.2009.12.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mycobacterium bovis BCG is still the most widely used vaccine against tuberculosis and CD8(+) T cells play important roles in fighting infection. We investigated how well antigen is processed and presented to CD8(+) T cells using the same well-characterized CD8(+) T cell epitope SIINFEKL expressed in either a cytoplasmic (GFP-OVA) or secreted (85B-OVA) context from BCG. We report that secreted SIINFEKL from 85B-OVA BCG is presented better than cytoplasmic SIINFEKL expressed by GFP-OVA BCG. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：36 / 42

页数：7

共 49 条

[1]

*2009 WR, 2009, GLOB TUB CONTR

[2] Early phagosomes in dendritic cells form a cellular compartment sufficient for cross presentation of exogenous antigens [J].

Ackerman, AL ;

Kyritsis, C ;

Tampé, R ;

Cresswell, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (22) :12889-12894

[3] A role for the endoplasmic reticulum protein retrotranslocation machinery during crosspresentation by dendritic cells [J].

Ackerman, Anne L. ;

Giodini, Alessandra ;

Cresswell, Peter .

IMMUNITY, 2006, 25 (04) :607-617

[4] The success and failure of BCG - implications for a novel tuberculosis vaccine [J].

Andersen, P ;

Doherty, TM .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (08) :656-662

[5] MHC Class I Endosomal and Lysosomal Trafficking Coincides with Exogenous Antigen Loading in Dendritic Cells [J].

Basha, Genc ;

Lizee, Gregory ;

Reinicke, Anna T. ;

Seipp, Robyn P. ;

Omilusik, Kyla D. ;

Jefferies, Wilfred A. .

PLOS ONE, 2008, 3 (09)

[6] Trafficking and release of mycobacterial lipids from infected macrophages [J].

Beatty, WL ;

Rhoades, ER ;

Ullrich, HJ ;

Chatterjee, D ;

Heuser, JE ;

Russell, DG .

TRAFFIC, 2000, 1 (03) :235-247

[7] Mycobacterial surface moieties are released from infected macrophages by a constitutive exocytic event [J].

Beatty, WL ;

Ullrich, HJ ;

Russell, DG .

EUROPEAN JOURNAL OF CELL BIOLOGY, 2001, 80 (01) :31-40

[8] Identification of mycobacterial surface proteins released into subcellular compartments of infected macrophages [J].

Beatty, WL ;

Russell, DG .

INFECTION AND IMMUNITY, 2000, 68 (12) :6997-7002

[9] Bacterial proteins can be processed by macrophages in a transporter associated with antigen processing-independent, cysteine protease-dependent manner for presentation by MHC class I molecules [J].

Campbell, DJ ;

Serwold, T ;

Shastri, N .

JOURNAL OF IMMUNOLOGY, 2000, 164 (01) :168-175

[10] Alternative processing for MHC class I presentation by immature and CpG-activated dendritic cells [J].

Chen, LY ;

Jondal, M .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2004, 34 (04) :952-960

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