The cytoplasmic male-sterile type and normal type mitochondrial genomes of sugar beet share the same complement of genes of known function but differ in the content of expressed ORFs

被引：117

作者：

Satoh, M ^{[1
]}

Kubo, T ^{[1
]}

Nishizawa, S ^{[1
]}

Estiati, A ^{[1
]}

Itchoda, N ^{[1
]}

Mikami, T ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Lab Genet Engn, Kita Ku, Sapporo, Hokkaido 0608589, Japan

来源：

MOLECULAR GENETICS AND GENOMICS | 2004年 / 272卷 / 03期

关键词：

chimeric gene; cytoplasmic male sterility; mitochondrial genome; Owen CMS; sugar beet;

D O I：

10.1007/s00438-004-1058-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete nucleotide sequence (501,020 bp) of the mitochondrial genome from cytoplasmic male-sterile (CMS) sugar beet was determined. This enabled us to compare the sequence with that previously published for the mitochondrial genome of normal, male-fertile sugar beet. The comparison revealed that the two genomes have the same complement of genes of known function. The rRNA and tRNA genes encoded in the CMS mitochondrial genome share 100% sequence identity with their respective counterparts in the normal genome. We found a total of 24 single nucleotide substitutions in 11 protein genes encoded by the CMS mitochondrial genome. However, none of these seems to be responsible for male sterility. In addition, several other ORFs were found to be actively transcribed in sugar beet mitochondria. Among these, Norf246 was observed to be present in the normal mitochondrial genome but absent from the CMS genome. However, it seems unlikely that the loss of Norf246 is causally related to the expression of CMS, because previous studies on mitochondrial translation products failed to detect the product of this ORF. Conversely, the CMS genome contains four transcribed ORFs (Satp6presequence, Scox2-2 , Sorf324 and Sorf119) which are missing from the normal genome. These ORFs, which are potential candidates for CMS genes, were shown to be generated by mitochondrial genome rearrangements.

引用

页码：247 / 256

页数：10

共 19 条

[1] The male sterile G cytoplasm of wild beet displays modified mitochondrial respiratory complexes
Ducos, E
Touzet, P
Boutry, M
[J]. PLANT JOURNAL, 2001, 26 (02) : 171 - 180
[2] The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria
Estiati, A
Kubo, T
Mikami, T
[J]. PHYSIOLOGIA PLANTARUM, 1998, 102 (02) : 325 - 327
[3] EXPRESSION OF THE WHEAT MITOCHONDRIAL NAD3-RPS12 TRANSCRIPTION UNIT - CORRELATION BETWEEN EDITING AND MESSENGER-RNA MATURATION
GUALBERTO, JM
BONNARD, G
LAMATTINA, L
GRIENENBERGER, JM
[J]. PLANT CELL, 1991, 3 (10) : 1109 - 1120
[4] VARIATION IN MITOCHONDRIAL TRANSLATION PRODUCTS IN FERTILE AND CYTOPLASMIC MALE-STERILE SUGAR-BEETS
HALLDEN, C
LIND, C
MOLLER, IM
[J]. THEORETICAL AND APPLIED GENETICS, 1992, 85 (2-3) : 139 - 145
[5] The sugar beet mitochondrial nad4 gene:: an intron loss and its phylogenetic implication in the Caryophyllales
Itchoda, N
Nishizawa, S
Nagano, H
Kubo, T
Mikami, T
[J]. THEORETICAL AND APPLIED GENETICS, 2002, 104 (2-3) : 209 - 213
[6] The rps4 gene in sugar beet mitochondria:: insertion/deletion mutations occur within the gene but do not disrupt the reading frame
Itchoda, N
Kubo, T
Estiati, A
Yamamoto, MP
Handa, H
Mikami, T
[J]. JOURNAL OF PLANT PHYSIOLOGY, 2002, 159 (02) : 211 - 215
[7] The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNACys(GCA)
Kubo, T
Nishizawa, S
Sugawara, A
Itchoda, N
Estiati, A
Mikami, T
[J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (13) : 2571 - 2576
[8] The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria
Kubo, T
Yamamoto, MP
Mikami, T
[J]. THEORETICAL AND APPLIED GENETICS, 2000, 100 (02) : 214 - 220
[9] Alterations in organization and transcription of the mitochondrial genome of cytoplasmic male sterile sugar beet (Beta vulgaris L.)
Kubo, T
Nishizawa, S
Mikami, T
[J]. MOLECULAR AND GENERAL GENETICS, 1999, 262 (02): : 283 - 290
[10] A duplicated sequence in sugarbeet mitochondrial transcripts is differentially edited: Analysis of orfB and its derivative orf324 mRNAs
Kubo, T
Mikami, T
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1996, 1307 (03): : 259 - 262

← 1 2 →