Adriamycin (ADM) induced apoptosis in transitional cell cancer (TCC) cell lines accompanied by p21(WAF1/CIP1) induction

被引：12
作者：
Bilim, VN
Tomita, Y
Kawasaki, T
Takeda, M
Takahashi, K
机构：
[1] Department of Urology, Niigata University, School of Medicine, Niigata 951
来源：
APOPTOSIS | 1997年 / 2卷 / 02期
关键词：
adriamycin (ADM); apoptosis; Bax; Bcl-2; p21(WAF1/CIP1); p53; transitional cell cancer (TCC); WILD-TYPE P53; DNA DAMAGE; DRUG-RESISTANCE; URINARY-TRACT; IRON COMPLEX; BCL-2; GENE; IN-VIVO; EXPRESSION; PROTEIN; DEATH;
D O I：
10.1023/A:1026472700554
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genotoxic stimuli, including anticancer drugs, induce apoptosis in cancer cells through increase of p53, p21(WAF1/CIP1), at least in part. Bcl-2 and Bax modify this pathway or directly regulated by p53. Here we studied Adriamycin (ADM)-induced apoptosis in four human bladder cancer cell lines-in respect of p53, p21(WAF1/CIP1) and Bcl-2 family proteins. After ADM, treatment bladder cancer cells underwent dose-dependent cell death with typical morphologic features of apoptosis. Among four cell lines RT4 with wt p53, low ratio of Bcl-2 to Bax and induction of p21(WAF1/CIP1) after ADM treatment, was the most sensitive to induction of apoptosis. Thus, p53, p21(WAF1/CIP1), Bcl-2 and Bax status might determine susceptibility of bladder cancer cells to ADM induced apoptosis.
引用
收藏
页码：207 / 213
页数：7
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